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Data and Publications
(A) The biological activity of Human Recombinant SDF-1 beta (CXCL12) was tested by its ability to mobilize Ca2+ in CHO-K1/Gα15/mCXCR4 cells (human Gα15 and mouse CXCR4 stably expressed in CHO-K1 cells). Ca2+ mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the example above is less than 2.5 μg/mL. (B) 5 μg of Human Recombinant SDF-1 beta (CXCL12) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant SDF-1 beta (CXCL12) has a predicted molecular mass of 8.5 kDa.