Mouse Recombinant IL-11

Interleukin 11

Mouse Recombinant IL-11

Interleukin 11

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Interleukin 11
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Overview

Interleukin 11 (IL-11) is a pleiotropic cytokine with effects on various tissues including the bone marrow, brain, and intestinal mucosa (Du & Williams). It belongs to the IL-6 family of cytokines that share a common signal transducer, gp130. Culture of mouse bone marrow cells with IL-11 in combination with IL-3, IL-6, and stem cell factor induces significant expansion and proliferation of colony-forming cells in vitro (Peters et al.). In addition, in combination with IL-3, IL-11 significantly enhances the growth of megakaryocytic colonies in vitro, suggesting its role in augmenting mouse megakaryopoiesis (Yonemura et al.). IL-11 is expressed in a wide range of normal adult mouse tissues, including the central nervous system, thymus, lung, and bone. The mouse IL-11 cDNA was cloned using an expression library generated from the lipopolysaccharide-induced mouse fetal thymic cell line, T2 (Morris et al.). The binding of IL-11 to its receptor induces heterodimerization with the gp130 subunit and activation of JAK tyrosine kinases. IL-11 also plays a role in cancer progression by inducing the proliferation of epithelial cancer cells and the survival of metastatic cells at distant organs. Recently, IL-11 has gained interest for its role in the pathogenesis of diseases in dysregulated mucosal homeostasis associated with STAT3 upregulation, including gastrointestinal cancers (Putoczki et al.).
Subtype
Cytokines
Cell Type
B Cells, Hematopoietic Stem and Progenitor Cells, Other, Pluripotent Stem Cells, T Cells
Species
Mouse
Area of Interest
Cancer, Immunology, Stem Cell Biology
Purity
≥ 95%

Data Figures

(A) The biological activity of Mouse Recombinant IL-11 was tested by its ability to promote the proliferation of B9 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 107 ng/mL. (B) 1 μg of Mouse Recombinant IL-11 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant IL-11 has a predicted molecular mass of 19.3 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78026, 78026.3, 78026.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78026, 78026.3, 78026.1
Lot #
All
Language
English

Applications

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