Mobilized Human Peripheral Blood Mononuclear Cells, Frozen

Primary human cells, frozen

Mobilized Human Peripheral Blood Mononuclear Cells, Frozen

Primary human cells, frozen

From: 233 USD
Catalog #
70049_C
Primary human cells, frozen

Overview

Start your assays with ready-to-use, ethically sourced, mobilized primary mononuclear cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications.

Donors are mobilized with:
• G-CSF: a maximum of 10 µg/kg/day of granulocyte colony-stimulating factor for 3 - 5 days prior to collection (Catalog #70049)
• Plerixafor: a maximum of 0.24 mg/kg plerixafor for 1 day prior to collection (Catalog #70074)
• G-CSF and Plerixafor: a maximum of 10 µg/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection (Catalog #70072)

Isolated from leukapheresis samples using density gradient separation or red blood cell lysis and cryopreserved in animal component-free CryoStor®CS10 medium (Catalog #07930), cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

Browse our Frequently Asked Questions (FAQs) on Primary Cells.
Contains
• CryoStor® CS10
Subtype
Frozen
Cell Type
Hematopoietic Stem and Progenitor Cells, Mononuclear Cells
Species
Human
Cell and Tissue Source
Peripheral Blood
Donor Status
Normal

Data Figures

Figure 1. Cryopreserved G-CSF Mobilized Human Peripheral Blood Mononuclear Cells Generate Hematopoietic Colonies in CFU Assays

Cryopreserved G-CSF Mobilized Human Peripheral Blood Mononuclear Cells (Catalog #70049) were thawed, plated at a concentration of 1 x 10^4 viable cells/dish and cultured in MethoCult™ Optimum Medium (Catalog #04034) for 14 days. Shown are colonies produced by hematopoietic progenitor cells within the sample, imaged with STEMvision™.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
70049, 70049.1, 70049.2, 70049.3, 70049.4
Lot #
All
Language
English
Catalog #
70072, 70072.1, 70072.2, 70072.3, 70072.4
Lot #
All
Language
English
Catalog #
70074, 70074.1, 70074.2, 70074.3, 70074.4
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Contact STEMCELL Technologies

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