Mobilized Human Peripheral Blood Mononuclear Cells, Frozen

Primary human cells, frozen
Catalog #
70049_C
Primary human cells, frozen
From: 203 USD

Overview

Start your assays with ready-to-use, ethically sourced, mobilized primary mononuclear cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications.

Donors are mobilized with:
• G-CSF: a maximum of 10 µg/kg/day of granulocyte colony-stimulating factor for 3 - 5 days prior to collection (Catalog #70049)
• Plerixafor: a maximum of 0.24 mg/kg plerixafore for 1 day prior to collection (Catalog #70074)
• G-CSF and Plerixafor: a maximum of 10 µg/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection (Catalog #70072)

Isolated from leukapheresis samples using density gradient separation or red blood cell lysis and cryopreserved in animal component-free CryoStor®CS10 medium (Catalog #07930), cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

Browse our Frequently Asked Questions (FAQs) on Primary Cells.
Contains
• CryoStor® CS10
Subtype
Frozen
Cell Type
Hematopoietic Stem and Progenitor Cells, Mononuclear Cells
Species
Human
Cell and Tissue Source
Peripheral Blood
Donor Status
Normal

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
G-CSF Mobilized Human Peripheral Blood Mononuclear Cells, Frozen
Catalog #
70049, 70049.1, 70049.2, 70049.3, 70049.4
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
G-CSF and Plerixafor Mobilized Human Peripheral Blood Mononuclear Cells, Frozen
Catalog #
70072, 70072.1, 70072.2, 70072.3, 70072.4
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Plerixafor Mobilized Human Peripheral Blood Mononuclear Cells, Frozen
Catalog #
70074, 70074.1, 70074.2, 70074.3, 70074.4
Lot #
All
Language
English

Educational Materials(11)

Brochure
STEMvision™ Automated and Standardized Counting of CFU Assays of Human Hematopoietic Cells
Brochure
Human Primary Cells
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays
Wallchart
Identification of Colonies Derived from Human Hematopoietic Progenitors
Wallchart
Human Hematopoietic Stem and Progenitor Cell Phenotyping
Video
6:05
How to Thaw Frozen Human Primary Cells
Webinar
58:45
Identification of Stem Cells in Myeloid Malignancies - Opportunities for Discovery and Translation
Webinar
53:35
Optimization and Standardization of the Colony-Forming Unit Assay for Hematopoietic Progenitor Cells
Webinar
50:50
Humanized Mouse Models for Hematopoietic Stem Cell Research: Principles and Pitfalls
Mini Review
Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Cryopreserved G-CSF Mobilized Human Peripheral Blood Mononuclear Cells Generate Hematopoietic Colonies in CFU Assays

Cryopreserved G-CSF Mobilized Human Peripheral Blood Mononuclear Cells (Catalog #70049) were thawed, plated at a concentration of 1 x 10^4 viable cells/dish and cultured in MethoCult™ Optimum Medium (Catalog #04034) for 14 days. Shown are colonies produced by hematopoietic progenitor cells within the sample, imaged with STEMvision™.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.