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Data and Publications
(A) The biological activity of Human Recombinant R-Spondin-1 was tested by its ability to induce luciferase activity in a WNT reporter HEK-293 cell line in the presence of mouse Wnt-3a. Luciferase activity was measured using a luminometric assay method. The EC50 is defined as the effective concentration of the growth factor at which luciferase activity is at 50% of maximum. The EC50 in the above example is 36.4 ng/mL. (B) Human Recombinant R-Spondin-1 was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant R-Spondin-1 has a predicted molecular mass of 25.6 kDa.