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Data and Publications
(A) The biological activity of Human Recombinant MIP-1 beta (CCL4) was tested by its ability to mobilize Ca2+ in CHO-K1/Gα15/hCCR5 cells (human Gα15 and human CCR5 stably expressed in CHO-K1 cells). Ca2+ mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the example above is less than 0.1 μg/mL. (B) 1 μg of Human Recombinant MIP-1 beta (CCL4) was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining. Human Recombinant MIP-1 beta (CCL4) has a predicted molecular mass of 7.6 kDa.