Human Recombinant IL-5, ACF

Interleukin 5, animal component-free

More Views

From: 167 USD

Options

* Required Fields

Catalog # (Select a product)
Interleukin 5, animal component-free
From: 167 USD

Overview

Interleukin 5 (IL-5) is a member of the short-chain 4-α-helical bundle subset of hematopoietic cytokines. It binds to a receptor consisting of IL-5Ra, which is specific for IL-5R and common beta chain, which is shared with the receptor for IL-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF; Martinez-Moczygemba & Huston). Upon binding to its receptor, IL-5 activates the JAK/STAT and MAPK pathways. IL-5 is produced by Th2 cells, eosinophils, and activated mast cells. It functions in the recruitment, activation, proliferation, and survival of eosinophils, thus playing an important role in allergic inflammation, asthma, and parasite immunity. Stimulation of eosinophils with IL-5 leads to their activation, upregulation of CD11b expression, and inhibition of apoptosis (Martinez-Moczygemba & Huston). This product is animal component-free.
Alternative Names:
B-cell differentiation factor I, EDF, Eosinophil colony-stimulating factor, Eosinophil differentiation factor, Interleukin-5, T-cell replacing factor, TRF
Cell Type:
B Cells; Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells
Species:
Human
Area of Interest:
Immunology; Stem Cell Biology
Molecular Weight:
13.3 kDa monomer; 26.6 kDa dimer
Purity:
≥ 95%

Scientific Resources

Product Documentation

Document Type
Product Name
Catalog #
Lot #
Language

Educational Materials

(4)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant IL-5, ACF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.166 ng/mL. (B) 1 μg of Human Recombinant IL-5, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-5, ACF has a predicted molecular mass of 26.6 kDa (13.3 kDa per monomer).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.