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Data and Publications
(A) The biological activity of Human Recombinant IHH was tested by its ability to induce alkaline phosphatase activity by CCL-226 cells. Alkaline phosphatase activity was measured by a fluorometric assay method. The EC50 is defined as the effective concentration of the signaling molecule at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the example above is less than 3 μg/mL. (B) 2 μg of Human Recombinant IHH was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IHH has a predicted molecular mass of 19.7 kDa.