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Data and Publications
(A) The biological activity of Human Recombinant GM-CSF was tested by its ability to promote the proliferation of TF-1 cells. Cell
proliferation was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective
concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.08 - 0.12 ng/mL.
(B) 1 μg of Human Recombinant GM-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant GM-CSF has a predicted molecular mass of 14.6 kDa.