Human/Mouse Recombinant FGF-8B, ACF

Fibroblast growth factor 8B, animal component-free

Human/Mouse Recombinant FGF-8B, ACF

Fibroblast growth factor 8B, animal component-free

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Fibroblast growth factor 8B, animal component-free
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Overview

Fibroblast growth factor 8B (FGF-8B) is a member of the fibroblast growth factor (FGF) family and is an isoform of FGF-8. Cytokines in the FGF family possess broad mitogenic and cell survival activities (Folkman & Klagsbrun; Kimelman & Kirschner) and are involved in a variety of biological processes, including cell proliferation, differentiation, survival, and apoptosis (Folkman & Klagsbrun; Klagsbrun; Rifkin & Moscatelli). FGF-8B signals through FGF receptors (FGFRs) to activate PI3K and MAPK pathways. FGF-8B regulates gastrulation, epithelial-mesenchymal transition, and mesenchymal to epithelial differentiation during embryonic development. FGF-8B has also been found in peripheral blood leukocytes and healthy bone marrow samples (Mattila & Härkönen). FGF-8B has mitogenic effects on somatic cells in the germinal epithelium and is expressed in adult mouse ovarian cells and tissues, which suggests that it regulates maturation of oocytes and seminiferous epithelium in testes (Valve et al.). This product is animal component-free.
Subtype
Cytokines, Growth Factors
Cell Type
Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Neural Cells, PSC-Derived, Pluripotent Stem Cells
Species
Human, Mouse
Area of Interest
Epithelial Cell Biology, Neuroscience, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 95%

Data Figures

(A) The biological activity of Human/Mouse Recombinant FGF-8B, ACF was tested by its ability to promote the proliferation of NR6R 3T3 cells in the presence of heparin. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.915 ng/mL. (B) 1 μg of Human/Mouse Recombinant FGF-8B, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human/Mouse Recombinant FGF-8B, ACF has a predicted molecular mass of 22.5 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78204, 78204.1, 78204.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78204, 78204.1, 78204.2
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.