Human Peripheral Blood Mononuclear Cells, Frozen

Primary human cells, frozen
From: 129 USD

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Primary human cells, frozen
From: 129 USD

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Overview

Primary human mononuclear cells (MNCs) were isolated from peripheral blood (PB) leukapheresis or whole blood samples using density gradient separation or red blood cell lysis. PB was collected using acid-citrate-dextrose solution A (ACDA), acid-citrate-dextrose solution B (ACDB), citrate-phosphate-dextrose (CPD), or sodium heparin (Na-Hep) as the anticoagulant.

Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.

Standard PBMCs (Catalog #70025) include the following characterization criteria: cell count, viability, donor virus testing, age, sex, ethnicity, weight, height and smoking status.

Characterized PBMCs (Catalog #70047) include the same information as Standard PBMCs in addition to the following characterization criteria: high-resolution HLA typing (A, B, C, DRB1, DRB3/4/5 and DQB1), ADCC status, ELISpot reactivity and cell proliferation index.

HLA-A2 PBMCs (Catalog #70048) are selected for HLA-A2 positive donors and include the same characterization criteria as Standard PBMCs and Characterized PBMCs.

Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.
Contains:
• CryoStor® CS10 (Catalog #70025) or
• Serum-free cryopreservation medium and 10% dimethyl sulfoxide (DMSO) (Catalog #70047 and #70048)
Subtype:
Frozen
Cell Type:
Mononuclear Cells
Species:
Human
Cell and Tissue Source:
Peripheral Blood
Donor Status:
Normal

Technical Resources

Product Documentation

Document Type
Product Name
Catalog #
Lot #
Language
70047, 70047.1, 70047.2
1000113144, 1000113148, 1000113152, 1000113160, 1000113162, 1000113167, 1500113159, 1500113163, 1500113164, 1500113165, 1500113149, 1250113159, 1250113163, 1250113164, 1250113165
70048, 70048.1, 70048.2
1000113150, 1000113151, 1000113153, 1500113156, 1500113166, 1500113168, 1250113156, 1250113166

Educational Materials

(2)

Data and Publications

Publications

(9)
Journal of biotechnology 2016 MAY

A novel approach for emerging and antibiotic resistant infections: Innate defense regulators as an agnostic therapy.

North JR et al.

Abstract

Innate Defense Regulators (IDRs) are short synthetic peptides that target the host innate immune system via an intracellular adaptor protein which functions at key signaling nodes. In this work, further details of the mechanism of action of IDRs have been discovered. The studies reported here show that the lead clinical IDR, SGX94, has broad-spectrum activity against Gram-negative and Gram-positive bacterial infections caused by intracellular or extracellular bacteria and also complements the actions of standard of care antibiotics. Based on in vivo and primary cell culture studies, this activity is shown to result from the primary action of SGX94 on tissue-resident cells and subsequent secondary signaling to activate myeloid-derived cells, resulting in enhanced bacterial clearance and increased survival. Data from non-clinical and clinical studies also show that SGX94 treatment modulates pro-inflammatory and anti-inflammatory cytokine levels, thereby mitigating the deleterious inflammatory consequences of innate immune activation. Since they act through host pathways to provide both broad-spectrum anti-infective capability as well as control of inflammation, IDRs are unlikely to be impacted by resistance mechanisms and offer potential clinical advantages in the fight against emerging and antibiotic resistant bacterial infections.
Analytical Chemistry 2016 APR

Water-in-Water Droplets by Passive Microfluidic Flow Focusing

Moon B-U et al.

Abstract

Org. Biomol. Chem. 2016

Cellular thermal shift and clickable chemical probe assays for the determination of drug-target engagement in live cells

Xu H et al.

Abstract

Frontiers in Cellular and Infection Microbiology 2015 DEC

Mycobacterium tuberculosis Infection Induces HDAC1-Mediated Suppression of IL-12B Gene Expression in Macrophages

Chandran A et al.

Abstract

Applied and Environmental Microbiology 2013 MAR

Identification of One Novel Candidate Probiotic Lactobacillus plantarum Strain Active against Influenza Virus Infection in Mice by a Large-Scale Screening

Kechaou N et al.

Abstract

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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