Human Peripheral Blood Effector Memory CD4+ T Cells, Frozen

Primary human cells, frozen

Product Note: This product is available at a discount while supplies last. Once supplies run out, you may request this item as a custom, made-to-order product. Browse our Primary Cells Custom Solutions for more information.

Human Peripheral Blood Effector Memory CD4+ T Cells, Frozen

Primary human cells, frozen

From: 2,915 USD
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Primary human cells, frozen
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Overview

Start experiments confidently with ready-to-use, ethically sourced primary human effector memory CD4+ T cells (CD3+CD4+CD45RO+CD62L-). With personalized service, custom products, flexible delivery times, and the option to reserve entire lots to pre-screen cells for applications, we help you get the cells you need.

Human effector memory CD4+ T cells are isolated from peripheral blood mononuclear cells (PBMCs) using an immunomagnetic depletion technique to remove CD62L-expressing cells, followed by negative immunomagnetic enrichment for CD4+ T cells, and cryopreserved in animal component-free CryoStor®CS10 medium. Blood samples are collected using Institutional Review Board (IRB) or other regulatory authority-approved consent forms and protocols. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant. Additional documentation to meet institutional requirements and high-resolution HLA typing (Class I and Class II alleles and CMV status) is available upon request. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

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Contains
CryoStor® CS10
Subtype
Frozen
Cell Type
T Cells, T Cells, CD4+
Species
Human
Cell and Tissue Source
Peripheral Blood
Area of Interest
Immunology
Donor Status
Normal
Purity
≥ 90% CD3+CD4+CD45RO+ by flow cytometry immediately after isolation, ≥ 85% CD62L-CD45RO+ (as a percentage of CD4+CD3+ cells) by flow cytometry after 24 hours in culture

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
200-0381
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Product Note: This product is available at a discount while supplies last. Once supplies run out, you may request this item as a custom, made-to-order product. Browse our Primary Cells Custom Solutions for more information.