Human pan IFN-alpha ELISA Kit

For detection and measurement of human pan interferon-alpha

Human pan IFN-alpha ELISA Kit

For detection and measurement of human pan interferon-alpha

From: 961 USD
Catalog #
02000_C
For detection and measurement of human pan interferon-alpha

What's Included

  • Human pan IFN-alpha ELISA Kit, 2 plates (Catalog #02000)
    • Human pan IFN-α ELISA Plate, 2x
    • Human pan IFN-α Standard, 1 vial
    • Human pan IFN-α Biotinylated Detection Antibodies, 30 µL
    • SA-HRP, 30 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 120 mL
    • ELISA Diluent, 120 mL
    • SA-HRP Diluent, 25 mL
    • TMB Substrate, 25 mL
    • Stop Solution, 25 mL
    • Adhesive Plate Covers, 6x
 
  • Human pan IFN-alpha ELISA Kit, 10 plates (Catalog #02001)
    • Human pan IFN-α ELISA Plate, 10x
    • Human pan IFN-α Standard, 1 vial
    • Human pan IFN-α Biotinylated Detection Antibodies, 130 µL
    • SA-HRP, 130 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 5 x 120 mL
    • ELISA Diluent, 3 x 120 mL
    • SA-HRP Diluent, 120 mL
    • TMB Substrate, 120 mL
    • Stop Solution, 120 mL
    • Adhesive Plate Covers, 30x

Overview

The Human pan Interferon-alpha (IFN-α) ELISA Kit is designed for the quantitative detection and measurement of human IFN-α in biological fluids such as serum, plasma, and cell culture supernatants. IFN-α is primarily produced by macrophages resulting from viral infections and activates anti-viral and immunomodulatory activities in target cells. IFN-α is secreted as multiple subtypes; this kit is specific for subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16, and 17.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Subtype
Complete Kits
Cell Type
Dendritic Cells, Macrophages
Species
Human
Application
ELISA
Area of Interest
Immunology

Data Figures

Representative Standard Curve

• Reportable range: 3.2 - 316 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC* international standard 95/566.
• Recovery: A mid-curve recovery of 92 - 123% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.7% (CV). The inter-assay precision of this assay is 10.0% (CV).

*National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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02000, 02001
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All
Language
English
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Safety Data Sheet 1
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02000, 02001
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English
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Safety Data Sheet 2
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Safety Data Sheet 3
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Safety Data Sheet 5
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02000, 02001
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Safety Data Sheet 6
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02000, 02001
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Safety Data Sheet 7
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Safety Data Sheet 8
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Safety Data Sheet 9
Catalog #
02000, 02001
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All
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English
Catalog #
02000, 02001
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All
Language
English
Catalog #
02000, 02001
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (2)

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