The Human Interleukin 7 (IL-7) ELISA Kit is designed for the quantitative detection and measurement of human IL-7 in cell culture supernatants. IL-7 is a hematopoietic growth factor that is secreted by stromal cells from the bone marrow and thymus. It signals through IL-7R, which stimulates the differentiation of hematopoietic stem cells to lymphoid progenitor cells. IL-7 is important for T cell homeostasis and promotes cell survival and proliferation of both naïve and memory T cells.

The assay is based on a sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for human IL-7. The captured IL-7 is detected by the addition of a horseradish peroxidase (HRP)-conjugated detection antibody. The addition of the chromogenic enzyme substrate 3,3’5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of human IL-7 in the sample.