Human IgE ELISA Antibody Pair Kit

For detection and measurement of human immunoglobulin E

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Human IgE ELISA Antibody Pair Kit

For detection and measurement of human immunoglobulin E

1 Kit for 6 Plates
Catalog #01993
375 USD

Required Products

Overview

The Human Immunoglobulin E (IgE) ELISA Antibody Pair Kit is intended for those who want the flexibility of setting up their own ELISA assay. This kit includes capture and detection antibodies and an IgE standard. It is designed for the quantitative detection and measurement of human IgE in biological fluids such as serum, plasma, and cell culture supernatants. IgE is predominantly found in the lungs, skin, and mucous membranes and is the least common immunoglobulin in serum. IgE plays a fundamental role in allergic reactions by stimulating degranulation of eosinophils, mast cells, and basophils through interaction with the Fc epsilon Receptor I (FcεRI).

The assay is based on the sandwich ELISA method, in which samples are added to ELISA plates coated with capture antibodies specific for the immunoglobulin. The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-alkaline phosphatase (SA-ALP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate p-nitrophenyl phosphate (pNPP) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample. The concentration of the immunoglobulin is determined by comparison to a serial dilution of the immunoglobulin standard analyzed in parallel.

NOTE: This kit includes sufficient reagents for 6 x 96-well ELISA plates. ELISA plates (Catalog #38019) and pNPP Substrate (Catalog #01917) are required for use with the Human IgE ELISA Antibody Pair Kit and are available for purchase separately. For serum or plasma samples, ELISA Diluent (Catalog #01916) is also required.
Components:
  • Human IgE Capture Antibody, 150 µL
  • Human IgE Biotinylated Detection Antibody, 80 µL
  • SA-ALP, 80 µL
  • Human IgE Standard, 1 vial
Subtype:
Antibody Pair Kits
Cell Type:
B Cells; Hybridomas
Species:
Human
Area of Interest:
Hybridoma Generation; Immunology

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Representative Standard Curve

• Reportable Range: 0.1 - 32 ng/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 75/502.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.