Human IgE ELISA Antibody Pair Kit

For detection and measurement of human immunoglobulin E
Human IgE ELISA Antibody Pair Kit

For detection and measurement of human immunoglobulin E

1 Kit for 6 Plates
Catalog # 01993
405 USD
New! For your convenience, all of our antibody pair kits now include a vial of Standard Reconstitution Buffer B. This change does not affect product performance or quality.
Required Products
  1. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

  2. Corning® 96-Well High-Binding Flat-Bottom Microplates
    Corning® 96-Well High-Binding Flat-Bottom Microplate

    Clear polystyrene flat-bottom, high-binding microplate without lid

  3. ELISA Diluent
    ELISA Diluent

    Diluent for ELISA samples, standards, and detection antibodies

  4. pNPP ELISA Substrate
    pNPP ELISA Substrate

    Substrate for ELISA antibody pair kits

Overview

The Human Immunoglobulin E (IgE) ELISA Antibody Pair Kit is intended for those who want the flexibility of setting up their own ELISA assay. This kit includes capture and detection antibodies and an IgE standard. It is designed for the quantitative detection and measurement of human IgE in biological fluids such as serum, plasma, and cell culture supernatants. IgE is predominantly found in the lungs, skin, and mucous membranes and is the least common immunoglobulin in serum. IgE plays a fundamental role in allergic reactions by stimulating degranulation of eosinophils, mast cells, and basophils through interaction with the Fc epsilon Receptor I (FcεRI).

The assay is based on the sandwich ELISA method, in which samples are added to ELISA plates coated with capture antibodies specific for the immunoglobulin. The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-alkaline phosphatase (SA-ALP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate p-nitrophenyl phosphate (pNPP) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample. The concentration of the immunoglobulin is determined by comparison to a serial dilution of the immunoglobulin standard analyzed in parallel.

NOTE: This kit includes sufficient reagents for 6 x 96-well ELISA plates. ELISA plates (Catalog #38019) and pNPP Substrate (Catalog #01917) are required for use with the Human IgE ELISA Antibody Pair Kit and are available for purchase separately. For serum or plasma samples, ELISA Diluent (Catalog #01916) is also required.
Components
  • Human IgE Capture Antibody, 150 µL
  • Human IgE Biotinylated Detection Antibody, 80 µL
  • SA-ALP, 80 µL
  • Human IgE Standard, 1 vial
  • Standard Reconstitution Buffer B, 1 mL
Subtype
Antibody Pair Kits
Cell Type
B Cells, Hybridomas
Species
Human
Area of Interest
Hybridoma Generation, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human IgE ELISA Antibody Pair Kit
Catalog #
01993
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Human IgE ELISA Antibody Pair Kit
Catalog #
01993
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Human IgE ELISA Antibody Pair Kit
Catalog #
01993
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Human IgE ELISA Antibody Pair Kit
Catalog #
01993
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Human IgE ELISA Antibody Pair Kit
Catalog #
01993
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Human IgE ELISA Antibody Pair Kit
Catalog #
01993
Lot #
All
Language
English

Educational Materials(2)

Brochure
ELISA Kits for Immunology Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Representative Standard Curve

• Reportable Range: 0.1 - 32 ng/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 75/502.

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