Human IFN-gamma ELISA Kit

For detection and measurement of human interferon-gamma
Catalog #
02002_C
For detection and measurement of human interferon-gamma
From: 768 USD

Overview

The Human Interferon-gamma (IFN-γ) ELISA Kit is designed for the quantitative detection and measurement of human IFN-γ in biological fluids such as serum, plasma, and cell culture supernatants. IFN-γ is primarily secreted by T and NK cells, but is also produced by dendritic cells and macrophages in smaller amounts. IFN-γ is a pleiotropic cytokine that modulates a broad range of immunological functions including antimicrobial and antitumor activity, and increases the surface expression of MHC class I and II proteins.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Components
  • Human IFN-gamma ELISA Kit, 2 plates (Catalog #02002)
    • Human IFN-γ ELISA Plate, 2x
    • Human IFN-γ Standard, 1 vial
    • Human IFN-γ Biotinylated Detection Antibody, 30 µL
    • SA-HRP, 30 µL
    • Standard Reconstitution Buffer C, 1 mL
    • Wash Buffer (20X), 120 mL
    • ELISA Diluent, 120 mL
    • SA-HRP Diluent, 25 mL
    • TMB Substrate, 25 mL
    • Stop Solution, 25 mL
    • Adhesive Plate Covers, 6x
 
  • Human IFN-gamma ELISA Kit, 10 plates (Catalog #02003)
    • Human IFN-γ ELISA Plate, 10x
    • Human IFN-γ Standard, 1 vial
    • Human IFN-γ Biotinylated Detection Antibody, 130 µL
    • SA-HRP, 130 µL
    • Standard Reconstitution Buffer C, 1 mL
    • Wash Buffer (20X), 5 x 120 mL
    • ELISA Diluent, 3 x 120 mL
    • SA-HRP Diluent, 120 mL
    • TMB Substrate, 120 mL
    • Stop Solution, 120 mL
    • Adhesive Plate Covers, 30x
Subtype
Complete Kits
Cell Type
Dendritic Cells, Macrophages, NK Cells, T Cells
Species
Human
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02002, 02003
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02002
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02002, 02003
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02002, 02003
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02002, 02003
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02002, 02003
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02002, 02003
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02002, 02003
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02002, 02003
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02002, 02003
Lot #
All
Language
English
Document Type
Safety Data Sheet 10
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02002, 02003
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Human IFN-gamma ELISA Kit
Catalog #
02003
Lot #
All
Language
English

Educational Materials (2)

Brochure
ELISA Kits for Immunology Research
Wallchart
Human Immune Cytokines

Product Applications

Data and Publications

Data

Representative Standard Curve

• Reportable range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIAID* international standard Gxg01-902-535.
• Recovery: A mid-curve recovery of 85 - 91% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.9% (CV). The inter-assay precision of this assay is 4.4% (CV).

*National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892-6612, USA.

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