Human Airway Epithelial Cell Training Course

Two-day hands-on training to support the culture of human airway epithelial cells at the air-liquid interface
Catalog #
00218_C
Two-day hands-on training to support the culture of human airway epithelial cells at the air-liquid interface
From: 1,250 USD
Note on COVID-19 restrictions: STEMCELL Technologies is following local governmental advice and will not conduct in-person training events until it is safe to do so. We are continually monitoring the situation and hope to resume our hands-on offerings soon. In the meantime, we continue to support your training needs with live virtual and on-demand training courses. For our current course availability, please see our training schedule.

Overview

Gain hands-on experience with the culture of human airway epithelial cells using the air-liquid interface (ALI) culture system. This system promotes extensive mucociliary differentiation of human airway epithelial cells to form a pseudostratified epithelium with morphological characteristics similar to those observed in the human airway in vivo. Participants will learn to expand primary human airway epithelial cells in PneumaCult™-Ex and PneumaCult™-Ex Plus Media, prepare cultureware for ALI culture, establish ALI cultures of human airway epithelial cells with PneumaCult™-ALI Medium, and perform downstream assays for airway epithelial cells at the ALI.

Participants Will Receive

  • Course booklet and electronic versions of instructional material
  • Tote bag with sample-size products (where applicable)
  • Certificate following successful completion of the course
  • Lunch and refreshments on each day and dinner hosted on one of the evenings
Training and Education
Training Programs
Disease Modeling; Drug Discovery and Toxicity Testing; Epithelial Cell Biology
Airway Cells
Human
Canada:
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6

Speakers

Juan Hou, PhD
Senior Scientist, Research & Development
STEMCELL Technologies
Guest Speaker: Details Coming Soon

Agenda

The course will end at approximately 5:00 pm on the first day and at 12:30 pm on the second day.


Day One

  • Welcome Presentation and Introductions
  • Lecture: Basic Biology and In Vitro Culture of Human Airway Epithelial Cells
  • Practical Session: Human Airway Epithelial Cell Morphology and Maintenance
    • Observe airway epithelial cells expanded in PneumaCult™ -Ex and PneumaCult™ -Ex Plus
    • Observe airway epithelial cells differentiated at the air-liquid interface (ALI)
    • Discuss routine maintenance of cultures
  • Practical Session: Demonstration of Preparing Airway Epithelial Cells for ALI
    • Demonstration of preparing cultureware
    • Demonstration of harvesting and seeding of airway epithelial cells onto transwell insert
  • Practical Session: Hands-On Practice with Preparing Airway Epithelial Cells for ALI
  • Lecture: Assays for Human Airway Epithelial Cells
  • Wrap Up and Q&A
  • Course Dinner

Day Two

  • Lecture: Research Applications for Human Airway Epithelial Cells
  • Practical Session: Assays for Airway Epithelial Cells at the ALI (Part I)
    • Demonstration & hands-on practice with transepithelial electrical resistance (TEER) readings
    • Demonstration of Ussing chamber to measure epithelial membrane properties
  • Practical Session: Assays for Airway Epithelial Cells at the ALI (Part II)
    • Demonstration and hands-on practice with OCT and agarose embedding for histological analysis
    • Demonstration of cell staining and fluorescence imaging
  • Wrap up Session:
    • Q&A
    • Quiz
    • Course Evaluation
    • Certificate Presentation

Accommodation

Vancouver, CA

Pan Pacific Vancouver
999 Canada Pl #300, Vancouver, BC, Canada, V6C 3B5
Tel 1-800-663-1515 or 1-604-662-3223
Sheraton Wall Centre
1088 Burrard Street, Vancouver, BC V6Z 2R9
Tel: 1-604-331-1000
Holiday Inn Vancouver-Centre
711 West Broadway, Vancouver, BC V5Z 3Y2
Tel: 1-604-879-0511
Executive Hotel LeSoleil
567 Hornby Street, Vancouver, BC V6C 2E8
Tel: 1-604-632-3000

Contact education@stemcell.com for information on special rates (when applicable).

Policy

Travel Visas

Citizens may require a visa in order to enter the country as visitors. Please contact us at education@stemcell.com if you require a letter of invitation for your application. Note that travel visas may take up to 30 days to process.

Travel Visa Requirements

Canada

Cancellation

A 90% refund will be issued if notification is received in writing more than 30 days in advance of the course start date. No refund will be given with less than 30 days’ notice unless we are able to find another participant to fill the spot.

STEMCELL Technologies reserves the right to cancel the course if we do not receive a minimum number of registrations 30 days in advance of the start date. Should the course be cancelled, your payment will be refunded in full. We recommend that you purchase travel insurance with your flights to cover the possibility of the course being cancelled.

Scientific Resources

Educational Materials (7)

Brochure
2019-2020 Cell Culture Training Catalog
Brochure
Modeling Cystic Fibrosis Airway: PneumaCult™-Ex Plus and PneumaCult™-ALI
Technical Bulletin
Air-Liquid Interface Culture for Respiratory Research
Video
Why Use Air-Liquid Interface Cultures for Respiratory Research: Q&A with Dr. Wadsworth
3:55
Why Use Air-Liquid Interface Cultures for Respiratory Research: Q&A with Dr. Wadsworth
Video
STEMCELL Technologies Inc. Training Programs
2:13
STEMCELL Technologies Inc. Training Programs
Video
Air-Liquid Interface (ALI) Culture of Bronchial Epithelial Cells with PneumaCult™-ALI Medium
2:01
Air-Liquid Interface (ALI) Culture of Bronchial Epithelial Cells with PneumaCult™-ALI Medium
Mini Review
Airway Epithelial Cells of the Human Respiratory System

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Contact STEMCELL Technologies

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