hPSC Genetic Analysis Kit
qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells
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hPSC Genetic Analysis Kit -
Label for hPSC Genetic Analysis Kit
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Required Products
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For purification of genomic DNA from cells or tissue
Overview
The hPSC Genetic Analysis Kit contains all required components to detect the 8 most common karyotypic abnormalities reported in human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. This qPCR-based kit enables the genetic screening of multiple human ES and iPS cell lines in a rapid and cost-effective manner, and contains enough material to analyze 20 individual samples in triplicate. It uses double-quenched probes with a 5-carboxyfluorescein (5-FAM) dye to give superior performance over other single-quenched probes.
Specifically, the kit includes qPCR Master Mix, ROX Reference Dye, Buffer and primer/probe mixes designed to detect the critical minimal regions of these 8 commonly mutated regions along with a primer/probe mix to a control location in the genome. Additionally, a genomic DNA control is provided with demonstrated normal copy number over the regions of interest.
Our online Genetic Analysis Application ( www.stemcell.com/geneticanalysisapp ) is designed to help with data analysis and interpretation: simply input qPCR data and the tool will perform statistical analyses, assist with data interpretation, and provide visual representation of the data.
Specifically, the kit includes qPCR Master Mix, ROX Reference Dye, Buffer and primer/probe mixes designed to detect the critical minimal regions of these 8 commonly mutated regions along with a primer/probe mix to a control location in the genome. Additionally, a genomic DNA control is provided with demonstrated normal copy number over the regions of interest.
Our online Genetic Analysis Application ( www.stemcell.com/geneticanalysisapp ) is designed to help with data analysis and interpretation: simply input qPCR data and the tool will perform statistical analyses, assist with data interpretation, and provide visual representation of the data.
Components:
• qPCR Master Mix (2X), 3 mL
• ROX Reference Dye, 0.2 mL
• Chr 1q Genetic Assay, 60 Rxn
• Chr 4p Genetic Assay, 60 Rxn
• Chr 8q Genetic Assay, 60 Rxn
• Chr 10p Genetic Assay, 60 Rxn
• Chr 12p Genetic Assay, 60 Rxn
• Chr 17q Genetic Assay, 60 Rxn
• Chr 18q Genetic Assay, 60 Rxn
• Chr 20q Genetic Assay, 60 Rxn
• Chr Xp Genetic Assay, 60 Rxn
• Genomic DNA Control, 15 µL
• TE Buffer, 1 mL
Cell Type:
Pluripotent Stem Cells
Species:
Human
Area of Interest:
Disease Modeling; Drug Discovery and Toxicity Testing; Stem Cell Biology
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STEMdiff™ Trilineage Differentiation Kit
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Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array
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Scientific Resources
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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data
Figure 1. The hPSC Genetic Analysis Kit Identifies Chromosome 12 Trisomy
Chromosome 12 trisomy in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.
Figure 2. The hPSC Genetic Analysis Kit Identifies Chromosome 1 Duplication via Unbalanced Translocation
Unbalanced rearrangement of chromosome 1 in the WLS-1C human iPS cell line in which an extra copy of the long (q) arm of chromosome 1 translocated to the short arm (p) of chromosome 21 was (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.
Figure 3. The hPSC Genetic Analysis Kit Identifies Chromosome 20q11.21 Duplication
Chromosome 20q duplication in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar), (B) undetected by G-banding, and (C) confirmed by fluorescent in situ hybridization using probes for 20p11 (green) and 20q11.21 (red).
Figure 4. The hPSC Genetic Analysis Kit Identifies Abnormalities in Cultures with Approximately 30% Mosaicism
Genetically normal WLS-1C human iPS cells were mixed in the indicated ratios with WLS-1C human iPS cells containing a chromosome 20q duplication. Cultures with approximately 30% genetically abnormal cells exhibit a significantly enriched population of 20q11.21 duplication (orange bars).
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.