Specifically, the kit includes qPCR Master Mix, ROX Reference Dye, Buffer and primer/probe mixes designed to detect the critical minimal regions of these 8 commonly mutated regions along with a primer/probe mix to a control location in the genome. Additionally, a genomic DNA control is provided with demonstrated normal copy number over the regions of interest.
Our online Genetic Analysis Application ( www.stemcell.com/geneticanalysisapp ) is designed to help with data analysis and interpretation: simply input qPCR data and the tool will perform statistical analyses, assist with data interpretation, and provide visual representation of the data.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Figure 1. The hPSC Genetic Analysis Kit Identifies Chromosome 12 Trisomy
Chromosome 12 trisomy in WLS-1C hiPS cell line is (A) detected using the hPSC Genetic Analysis Kit and (B) confirmed by G-banding.
Figure 2. The hPSC Genetic Analysis Kit Identifies Chromosome 1q Duplication via Translocation
Unbalanced rearrangement of chromosome 1 identified in the WLS-1C iPS cell line in which an extra copy of the long (q) arm of chromosome 1 has been translocated to the short arm (p) of chromosome 21 (A) detected using the hPSC Genetic Analysis Kit and (B) confirmed by G-banding.
Figure 3. The hPSC Genetic Analysis Kit Identifies Chromosome 20q11.21 Duplication
Chromosome 20q duplication in STiPS-4D1 hiPS cell line is (A) detected using the hPSC Genetic Analysis Kit, (B) undetected by G-banding but (C) confirmed by fluorescent in situ hybridization using probes for 20p11 (green) and 20q11.21 (red).