ErythroClear™ Red Blood Cell Depletion Kit

Red blood cell depletion kit for small volume cord blood samples

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ErythroClear™ Red Blood Cell Depletion Kit

Kit including ErythroClear™ magnet and reagent

2 mL cord blood
Catalog #01739
2,543 USD

ErythroClear™ Red Blood Cell Depletion Reagent Kit

Additional reagent for use with an ErythroClear™ magnet

2 mL cord blood
Catalog #01738
74 USD

Overview

ErythroClear™ is intended for the depletion of red blood cells (RBCs) from up to 16 small volume (50 - 100 µL) fresh or frozen cord blood samples at a time. ErythroClear™ reagent contains immunomagnetic particles that bind to Glycophorin AB+ cells, which are then selectively depleted using an ErythroClear™ magnet. This product is recommended for the depletion of RBCs in cord blood samples prior to downstream assays (e.g. CFU assay, ALDHbr assay).

The ErythroClear™ Red Blood Cell Depletion Kit (Catalog #01739) contains an ErythroClear™ magnet and ErythroClear™ reagent. The ErythroClear™ magnet is not sold separately and is only available when purchased as a part of this kit.

The ErythroClear™ Red Blood Cell Depletion Reagent Kit (Catalog #01738) contains ErythroClear™ reagent only, and is suitable as a refill for labs that already have an ErythroClear™ magnet. The ErythroClear™ magnet is required for use of the ErythroClear™ reagent.
Advantages:
• Requires only 2 minutes per sample
• Significantly more effective in depleting RBCs than gravity sedimentation methods
• Compatible with downstream assays (e.g. CFU assay, CD34, ALDEFLUOR™ [Catalog #01700], and ALDECOUNT™)
• Does not alter the frequency of CFUs, CD34+, or ALDHbr cells
Components:
  • ErythroClear™ Red Blood Cell Depletion Kit (Catalog #01739)
    • ErythroClear™ reagent, 2 x 2 mL
    • ErythroClear™ magnet
  • ErythroClear™ Red Blood Cell Depletion Reagent Kit (Catalog #01738)
    • ErythroClear™ reagent, 2 x 2 mL
Subtype:
Cell Isolation Kits
Cell Type:
Hematopoietic Stem and Progenitor Cells; Red Blood Cells
Species:
Human
Sample Source:
Cord Blood
Selection Method:
Depletion
Application:
Cell Isolation
Brand:
ErythroClear
Area of Interest:
Cord Blood Banking; Stem Cell Biology

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

RBC Depletion of Whole Cord Blood Sample Using ErythroClear™

Figure 1. RBC Depletion of Whole Cord Blood Sample Using ErythroClear™

Representative Flow Cytometry Plots of Whole Cord Blood Samples Stained for GlyAB and CD45 Before and After RBC Depletion with ErythroClear™

Improved Visualization of Cord Blood-Derived Colonies in CFU Assays After RBC Depletion with ErythroClear™

Figure 2. Improved Visualization of Cord Blood-Derived Colonies in CFU Assays After RBC Depletion with ErythroClear™

Representative STEMvision™ images of cord blood CFU assays (A) with and (B) without ErythroClear™ RBC depletion prior to plating. The expanded orange circles illustrate that it is easier to identify small or diffuse colonies in MethoCult™ medium that is free of RBC background (A vs. B).

ErythroClear™ Does Not Change the Frequency of CFUs in Cord Blood Samples

Figure 3. ErythroClear™ Does Not Change the Frequency of CFUs in Cord Blood Samples

The frequency of CFUs in Erythroclear™ depleted (y-axis) and non-depleted (x-axis) cord blood samples are shown. One half of each cord blood sample was processed with ErythroClear™ prior to plating in MethoCult™ H4434 Classic medium. The other half of each sample was plated without RBC depletion. The total number of CFUs in the RBC depleted and non-depleted samples were scored manually after 14 days of culture (n = 32 fresh (triangle) and 8 frozen (circle) cord blood samples, solid line = linear regression best-fit, dotted lines define 95% confidence intervals). The frequency of CFUs in each sample before and after removal of RBCs using ErythroClear™ is highly correlated (correlation coefficient R2 = 0.83). The average recovery of total nucleated cells (TNCs) after RBC depletion with ErythroClear™ was 75%.

The Frequency of CD34+ Cells in Cord Blood is Not Changed After RBC Depletion with ErythroClear™

Figure 4. The Frequency of CD34+ Cells in Cord Blood is Not Changed After RBC Depletion with ErythroClear™

Shown are the percentages of GlyAB-CD45+ cells that are CD34+ in cord blood samples before (light gold) and after (gold) RBC depletion with ErythroClear™. There was no significant difference in the frequency of CD34+ cells in this population before and after RBC depletion for each sample (p>0.01). The recovery of CD34+ cells after RBC depletion with ErythroClear™ was 70%. Additionally the frequency of GlyAB-CD45+ that are ALDHbright (data not shown) was not significantly different after ErythroClear™ treatment (p>0.01), and the recovery of these cells was 66%. (n = 8 frozen whole cord blood samples, processed and analyzed separately in triplicate, columns with error bars represent mean ± S.D.).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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