EC-Cult™-XF Culture Kit

Culture kit for derivation and proliferation of human endothelial colony-forming cells (ECFCs) and mature endothelial cells

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EC-Cult™-XF Culture Kit

Culture kit for derivation and proliferation of human endothelial colony-forming cells (ECFCs) and mature endothelial cells

1 Kit
Catalog #08000
299 USD

Required Products

Overview

EC-Cult™-XF Culture Kit is a xeno-free medium for the culture of mature human endothelial cells and endothelial colony-forming cells (ECFCs). It has been developed for the expansion of vascular (large, small, and lymphatic vessel-derived endothelial cells) and perivascular cells (human placental pericytes), as well as for the derivation and proliferation of ECFCs from human umbilical cord blood and peripheral blood. For complete instructions for the ECFC assay, refer to the Technical Manual: Culture of Human Endothelial Colony-Forming Cells (ECFCs) Using EC‑Cult™-XF Culture Kit (Document #DX21400), available at www.stemcell.com or contact us to request a copy.

EC Cult™ XF Medium must be used in conjunction with Animal Component-Free Cell Attachment Substrate (Component #07130; included in EC-Cult™-XF Culture Kit). For passaging, Animal Component-Free Cell Dissociation Kit (Catalog #05426) is required. EC-Cult™-XF Medium must be supplemented with Heparin Solution (Catalog #07980). Since Heparin Solution contains non-human animal-derived components, the complete medium will not be xeno-free.
Advantages:
• Supports the robust expansion of large, small, and lymphatic vessel endothelial cells
• Serum-free formulation supports the derivation, expansion, and maintenance of ECFCs
• Rigorous raw material screening and quality control minimize lot-to-lot variability and increase reproducibility between experiments
Components:
  • EC-Cult™-XF Basal Medium, 150 mL
  • EC-Cult™-XF 2.5X Supplement, 100 mL
  • Animal Component-Free Cell Attachment Substrate, 1 mL
Subtype:
Specialized Media
Cell Type:
Endothelial Cells; Angiogenic Cells
Species:
Human
Application:
Cell Culture
Brand:
EC-Cult
Area of Interest:
Stem Cell Biology; Endothelial Cell Biology; Angiogenic Cell Research
Formulation:
Xeno-Free

Scientific Resources

Educational Materials

(2)

Data and Publications

Data

Publications

(2)
Stem cells translational medicine 2017 MAY

Endothelial Progenitors: A Consensus Statement on Nomenclature.

Medina RJ et al.

Abstract

Endothelial progenitor cell (EPC) nomenclature remains ambiguous and there is a general lack of concordance in the stem cell field with many distinct cell subtypes continually grouped under the term EPC." It would be highly advantageous to agree on standards to confirm an endothelial progenitor phenotype and this should include detailed immunophenotyping potency assays and clear separation from hematopoietic angiogenic cells which are not endothelial progenitors. In this review we seek to discourage the indiscriminate use of "EPCs and instead propose precise terminology based on defining cellular phenotype and function. Endothelial colony forming cells and myeloid angiogenic cells are examples of two distinct and well-defined cell types that have been considered EPCs because they both promote vascular repair, albeit by completely different mechanisms of action. It is acknowledged that scientific nomenclature should be a dynamic process driven by technological and conceptual advances; ergo the ongoing EPC" nomenclature ought not to be permanent and should become more precise in the light of strong scientific evidence. This is especially important as these cells become recognized for their role in vascular repair in health and disease and in some cases progress toward use in cell therapy. Stem Cells Translational Medicine 2017;6:1316-1320.
JCI insight 2017 JAN

CD44 expression in endothelial colony-forming cells regulates neurovascular trophic effect.

S. Sakimoto et al.

Abstract

Vascular abnormalities are a common component of eye diseases that often lead to vision loss. Vaso-obliteration is associated with inherited retinal degenerations, since photoreceptor atrophy lowers local metabolic demands and vascular support to those regions is no longer required. Given the degree of neurovascular crosstalk in the retina, it may be possible to use one cell type to rescue another cell type in the face of severe stress, such as hypoxia or genetically encoded cell-specific degenerations. Here, we show that intravitreally injected human endothelial colony-forming cells (ECFCs) that can be isolated and differentiated from cord blood in xeno-free media collect in the vitreous cavity and rescue vaso-obliteration and neurodegeneration in animal models of retinal disease. Furthermore, we determined that a subset of the ECFCs was more effective at anatomically and functionally preventing retinopathy; these cells expressed high levels of CD44, the hyaluronic acid receptor, and IGFBPs (insulin-like growth factor-binding proteins). Injection of cultured media from ECFCs or only recombinant human IGFBPs also rescued the ischemia phenotype. These results help us to understand the mechanism of ECFC-based therapies for ischemic insults and retinal neurodegenerative diseases.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.