EasySep™ Rat IgM Depletion Kit

Immunomagnetic depletion kit

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From: 839 USD

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Immunomagnetic depletion kit
From: 839 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Rat IgM Depletion Kit is designed to deplete IgM+ cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by positive selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages:
• Fast, easy-to-use and column-free
• Up to 90% purity (≤ 10% IgM+)
• Isolated cells are untouched
Components:
  • EasySep™ Rat IgM Depletion Kit (Catalog #18644)
    • EasySep™ Rat IgM Depletion Cocktail, 1 mL
    • Anti-Rat CD32 Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
  • RoboSep™ Rat IgM Depletion Kit (Catalog #18644RF)
    • EasySep™ Rat IgM Depletion Cocktail, 1 mL
    • Anti-Rat CD32 Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
• Easy50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells
Species:
Rat
Sample Source:
Lymph Node; Other; Spleen; Whole Blood
Selection Method:
Depletion
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(8)
Brochure
Fully Automated Cell Isolation with RoboSep™
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Immune Cells in Rat Tissue
Video
1:23
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
B Cell Research
Cell Sourcing
Cell Isolation
Cell Expansion
Cell Characterization

Data and Publications

Data

Starting with a single-cell suspension of splenocytes from a Sprague Dawley rat, the IgM content of the final isolated fraction is typically 8.0 ± 4.1%. In the above example, the purities of the start and final depleted fractions are 55.7% and 8.7%, respectively (1.6 log depletion).

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.