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EasySep™ Rat IgM Depletion Kit

Immunomagnetic depletion of rat IgM+ cells from single-cell suspensions of rat splenocytes, lymph nodes, whole blood, or other tissue samples

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Rat IgM Depletion Kit

Immunomagnetic depletion of rat IgM+ cells from single-cell suspensions of rat splenocytes, lymph nodes, whole blood, or other tissue samples

From: 989 USD
Catalog #
(Select a product)
Immunomagnetic depletion of rat IgM+ cells from single-cell suspensions of rat splenocytes, lymph nodes, whole blood, or other tissue samples
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Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 90% purity (≤ 10% IgM+)

  • Isolated cells are untouched

What's Included

  • EasySep™ Rat IgM Depletion Kit (Catalog #18644)
    • EasySep™ Rat IgM Depletion Cocktail, 1 mL
    • Anti-Rat CD32 Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
  • RoboSep™ Rat IgM Depletion Kit (Catalog #18644RF)
    • EasySep™ Rat IgM Depletion Cocktail, 1 mL
    • Anti-Rat CD32 Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer for use with EasySep™ cell separation kits

Overview

Efficiently deplete rat IgM+ cells from single-cell suspensions of rat splenocytes, lymph nodes, whole blood, or other tissue samples, using immunomagnetic positive selection, with the EasySep™ Rat IgM Depletion Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

This straightforward, optimized EasySep™ procedure involves labeling unwanted cells with antibodies recognizing specific cell surface markers and magnetic particles. Labeled cells are separated from untouched cells using an EasySep™ magnet and by simply pouring or pipetting off the unlabeled cells. The desired cells remain in the tube. Following magnetic cell isolation, desired cells are ready for downstream applications, such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
• Easy50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Rat
Sample Source
Lymph Node, Other, Spleen, Whole Blood
Selection Method
Depletion
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Starting with a single-cell suspension of splenocytes from a Sprague Dawley rat, the IgM content of the final isolated fraction is typically 8.0 ± 4.1%. In the above example, the purities of the start and final depleted fractions are 55.7% and 8.7%, respectively (1.6 log depletion).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (8)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Fully Automated Cell Isolation with RoboSep™
Brochure
Fully Automated Cell Isolation with RoboSep™
Frequencies of Immune Cells in Rat Tissue
Wallchart
Frequencies of Immune Cells in Rat Tissue
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument

Related Products

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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