EasySep™ Rat IgM Depletion Kit
Immunomagnetic depletion of rat IgM+ cells from single-cell suspensions of rat splenocytes, lymph nodes, whole blood, or other tissue samples

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In-lab demonstration
- EasySep™ Magnet
Magnet for column-free immunomagnetic separation
- EasySep™ Buffer
Cell separation buffer for use with EasySep™ cell separation kits
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Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells
Overview
This straightforward, optimized EasySep™ procedure involves labeling unwanted cells with antibodies recognizing specific cell surface markers and magnetic particles. Labeled cells are separated from untouched cells using an EasySep™ magnet and by simply pouring or pipetting off the unlabeled cells. The desired cells remain in the tube. Following magnetic cell isolation, desired cells are ready for downstream applications, such as flow cytometry, culture, or DNA/RNA extraction.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Data Figures

Starting with a single-cell suspension of splenocytes from a Sprague Dawley rat, the IgM content of the final isolated fraction is typically 8.0 ± 4.1%. In the above example, the purities of the start and final depleted fractions are 55.7% and 8.7%, respectively (1.6 log depletion).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (8)
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EasySep™ Rat IgM Depletion Kit
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.