EasySep™ Rat IgM Depletion Kit

Immunomagnetic depletion kit
Catalog #
18644_C
Immunomagnetic depletion kit
From: 856 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Rat IgM Depletion Kit is designed to deplete IgM+ cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by positive selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages
• Fast, easy-to-use and column-free
• Up to 90% purity (≤ 10% IgM+)
• Isolated cells are untouched
Components
  • EasySep™ Rat IgM Depletion Kit (Catalog #18644)
    • EasySep™ Rat IgM Depletion Cocktail, 1 mL
    • Anti-Rat CD32 Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
  • RoboSep™ Rat IgM Depletion Kit (Catalog #18644RF)
    • EasySep™ Rat IgM Depletion Cocktail, 1 mL
    • Anti-Rat CD32 Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
• Easy50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Rat
Sample Source
Lymph Node, Other, Spleen, Whole Blood
Selection Method
Depletion
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English

Educational Materials (8)

Brochure
EasySep™ Cell Separation Technology
Brochure
Fully Automated Cell Isolation with RoboSep™
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Immune Cells in Rat Tissue
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with a single-cell suspension of splenocytes from a Sprague Dawley rat, the IgM content of the final isolated fraction is typically 8.0 ± 4.1%. In the above example, the purities of the start and final depleted fractions are 55.7% and 8.7%, respectively (1.6 log depletion).

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