EasySep™ Rat IgM Depletion Kit
Immunomagnetic depletion kit
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EasySep™ Rat IgM Depletion Kit -
RoboSep™ Rat IgM Depletion Kit -
Label for EasySep™ Rat IgM Depletion Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer
Overview
The EasySep™ Rat IgM Depletion Kit is designed to deplete IgM+ cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by positive selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages:
• Fast, easy-to-use and column-free
• Up to 90% purity (≤ 10% IgM+)
• Isolated cells are untouched
• Up to 90% purity (≤ 10% IgM+)
• Isolated cells are untouched
Components:
EasySep™ Rat IgM Depletion Kit (Catalog #18644)
• EasySep™ Rat IgM Depletion Cocktail, 1 mL
• Anti-Rat CD32 Blocker, 0.1 mL
• EasySep™ Dextran RapidSpheres™ 50102, 1 mL
RoboSep™ Rat IgM Depletion Kit (Catalog #18644RF)
• EasySep™ Rat IgM Depletion Cocktail, 1 mL
• Anti-Rat CD32 Blocker, 0.1 mL
• EasySep™ Dextran RapidSpheres™ 50102, 1 mL
• RoboSep™ Buffer (Catalog #20104)
• RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
• Easy50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells
Species:
Rat
Sample Source:
Lymph Node; Other; Spleen; Whole Blood
Selection Method:
Depletion
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology
Related Products
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EasySep™ Rat T Cell Isolation Kit
Immunomagnetic negative selection kit -
EasySep™ Rat B Cell Isolation Kit
Immunomagnetic negative selection kit -
Tube for density gradient centrifugation for in vitro diagnostic (IVD) applications -
Tube for density gradient centrifugation for in vitro diagnostic (IVD) applications
Scientific Resources
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data and Publications
Data
Starting with a single-cell suspension of splenocytes from a Sprague Dawley rat, the IgM content of the final isolated fraction is typically 8.0 ± 4.1%. In the above example, the purities of the start and final depleted fractions are 55.7% and 8.7%, respectively (1.6 log depletion).
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.