EasySep™ Rat CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

Platform

EasySep RoboSep

Catalog #

19643_C

Immunomagnetic negative selection kit

From: 583 EUR

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EasySep™ Magnet
Magnet for column-free immunomagnetic separation
EasySep™ Buffer
Cell separation buffer
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

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Overview

The EasySep™ Rat CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

• Fast, easy-to-use and column-free
• Up to 94% purity
• Isolated cells are untouched

EasySep™ Rat CD8+ T Cell Isolation Kit (Catalog #19643)

EasySep™ Rat CD8+ T Cell Isolation Cocktail, 1 mL
EasySep™ Dextran RapidSpheres™ 50102, 1 mL

RoboSep™ Rat CD8+ T Cell Isolation Kit (Catalog #19643RF)

EasySep™ Rat CD8+ T Cell Isolation Cocktail, 1 mL
EasySep™ Dextran RapidSpheres™ 50102, 1 mL
RoboSep™ Buffer (Catalog #20104)
RoboSep™ Filter Tips (Catalog #20125)

• EasySep™ Magnet (Catalog #18000)

• “The Big Easy” EasySep™ Magnet (Catalog #18001)

• Easy 50 EasySep™ Magnet (Catalog #18002)

• EasyEights™ EasySep™ Magnet (Catalog #18103)

• RoboSep™-S (Catalog #21000)

Cell Isolation Kits

T Cells, T Cells, CD8+

Rat

Other, Spleen, Whole Blood

Negative

Cell Isolation

EasySep, RoboSep

Immunology

Scientific Resources

Product Documentation

Document Type	Product Name	Catalog #	Lot #	Language
Document Type Product Information Sheet	Product Name EasySep™ Rat CD8+ T Cell Isolation Kit	Catalog # 19643	Lot # All	Language English
Document Type Product Information Sheet	Product Name RoboSep™ Rat CD8+ T Cell Isolation Kit	Catalog # 19643RF	Lot # All	Language English
Document Type Safety Data Sheet 1	Product Name EasySep™ Rat CD8+ T Cell Isolation Kit	Catalog # 19643	Lot # All	Language English
Document Type Safety Data Sheet 2	Product Name EasySep™ Rat CD8+ T Cell Isolation Kit	Catalog # 19643	Lot # All	Language English
Document Type Safety Data Sheet 1	Product Name RoboSep™ Rat CD8+ T Cell Isolation Kit	Catalog # 19643RF	Lot # All	Language English
Document Type Safety Data Sheet 2	Product Name RoboSep™ Rat CD8+ T Cell Isolation Kit	Catalog # 19643RF	Lot # All	Language English

Educational Materials (8)

Brochure

Tools For Your Immunology Research

Brochure

EasySep™ Cell Separation Technology

Brochure

Fully Automated Cell Isolation with RoboSep™

Wallchart

Human Immune Cytokines

Wallchart

Frequencies of Immune Cells in Rat Tissue

Video

0:57

Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet

Video

1:41

Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument

Video

1:57

How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Frequently Asked Question

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for T Cell Research

Cell Sourcing

Cell Isolation

Cell Activation, Expansion, and Differentiation

Cell Characterization

Data and Publications

Data

Figure 1. Typical EasySep™ Rat CD8+ T Cell Isolation Kit Profile

Starting with rat splenocytes, the CD8+ T cell content (CD3+CD8+) of the isolated fraction is typically 91.5 ± 3.0% (mean ± SD using the purple EasySep™ Magnet).

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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EasySep™ Rat CD8+ T Cell Isolation Kit

Overview

Related Products

Scientific Resources

Product Documentation

Educational Materials (8)

Frequently Asked Question

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Product Applications

Data and Publications

Data

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