EasySep™ Rat CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

Skip to the beginning of the images gallery

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Rat CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

From: 709 USD

Platform

RoboSep EasySep

Catalog #

19643_C

Immunomagnetic negative selection kit

Quantity

Add to Wish List

Product Advantages

Fast, easy-to-use and column-free

Up to 94% purity

Isolated cells are untouched

What's Included

EasySep™ Rat CD8+ T Cell Isolation Kit (Catalog #19643)

EasySep™ Rat CD8+ T Cell Isolation Cocktail, 1 mL
EasySep™ Dextran RapidSpheres™ 50102, 1 mL

RoboSep™ Rat CD8+ T Cell Isolation Kit (Catalog #19643RF)

EasySep™ Rat CD8+ T Cell Isolation Cocktail, 1 mL
EasySep™ Dextran RapidSpheres™ 50102, 1 mL
RoboSep™ Buffer (Catalog #20104)
RoboSep™ Filter Tips (Catalog #20125)

Request Pricing

Thank you for your interest in this product. Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):

Estimated delivery time for your area

Product sample or exclusive offer

In-lab demonstration

By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.

To see all required products for your protocol, please consult the Protocols and Documentation.

EasySep™ Magnet
Magnet for column-free immunomagnetic separation
EasySep™ Buffer
Cell separation buffer
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

Overview

The EasySep™ Rat CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

• EasySep™ Magnet (Catalog #18000)

• “The Big Easy” EasySep™ Magnet (Catalog #18001)

• Easy 50 EasySep™ Magnet (Catalog #18002)

• EasyEights™ EasySep™ Magnet (Catalog #18103)

• RoboSep™-S (Catalog #21000)

Cell Isolation Kits

T Cells, T Cells, CD8+

Rat

Other, Spleen, Whole Blood

Negative

Cell Isolation

EasySep, RoboSep

Immunology

Data Figures

Figure 1. Typical EasySep™ Rat CD8+ T Cell Isolation Kit Profile

Starting with rat splenocytes, the CD8+ T cell content (CD3+CD8+) of the isolated fraction is typically 91.5 ± 3.0% (mean ± SD using the purple EasySep™ Magnet).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

EasySep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643

Lot #

All

Language

English

Document Type

Product Information Sheet

Product Name

RoboSep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

EasySep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

EasySep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

RoboSep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

RoboSep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 3

Product Name

RoboSep™ Rat CD8+ T Cell Isolation Kit

Catalog #

19643RF

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for T Cell Research

Cell Sourcing

Cell Isolation

Cell Activation, Expansion, and Differentiation

Cell Characterization

Resources and Publications

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Related Products

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

EasySep™ Rat CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

EasySep™ Rat CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

Product Advantages

What's Included

Request Pricing

Overview

Data Figures

Protocols and Documentation

Applications

Resources and Publications

Educational Materials (8)

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Related Products

Item added to your cart