EasySep™ Rat B Cell Isolation Kit

Immunomagnetic negative selection kit
Catalog #
19644_C
Immunomagnetic negative selection kit
From: 684 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Rat B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages
• Fast, easy-to-use and column-free
• Up to 95% purity
• Isolated cells are untouched
Components
EasySep™ Rat B Cell Isolation Kit (Catalog #19644)
    • EasySep™ Rat B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
RoboSep™ Rat B Cell Isolation Kit (Catalog #19644RF)
    • EasySep™ Rat B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• ”The BigEasy” EasySep™ Magnet (Catalog #18001)
• Easy50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Rat
Sample Source
Other, Spleen, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Rat B Cell Isolation Kit
Catalog #
19644
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Rat B Cell Isolation Kit
Catalog #
19644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Rat B Cell Isolation Kit
Catalog #
19644
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Rat B Cell Isolation Kit
Catalog #
19644
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Rat B Cell Isolation Kit
Catalog #
19644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Rat B Cell Isolation Kit
Catalog #
19644RF
Lot #
All
Language
English

Educational Materials (8)

Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Brochure
Fully Automated Cell Isolation with RoboSep™
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Immune Cells in Rat Tissue
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument

Data and Publications

Data

19644 FACS

Starting with a single-cell suspension of rat splenocytes, the B cell content (CD45RA+CD3-) of the isolated fraction is typically 94.1 ± 3.8% (mean ± SD using the purple EasySep™ Magnet). In the example above, the purities of the start and final isolated fractions are 44.5% and 95.6%, respectively.

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