EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit

25-Minute cell isolation kit using immunomagnetic negative selection

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25-Minute cell isolation kit using immunomagnetic negative selection

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Overview

The EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This kit has been verified for use with rhesus and cynomolgus macaques.
Advantages:
⦁ Fast, easy to use, and column-free
⦁ Up to 85% purity with high recovery
⦁ Untouched, viable cells
Components:
  • EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit (Catalog #19583)
    • EasySep™ Non-Human Primate CD8+ T Cell Isolation Cocktail, 1.2 mL
    • EasySep™ D Magnetic Particles, 3 x 1 mL
  • RoboSep™ Non-Human Primate CD8+ T Cell Isolation Kit (Catalog #19583RF)
    • EasySep™ Non-Human Primate CD8+ T Cell Isolation Cocktail, 1.2 mL
    • EasySep™ D Magnetic Particles, 3 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Cell Type:
T Cells, CD8+
Species:
Non-Human Primate
Sample Source:
PBMC; Peripheral Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
HIV; Immunology; Infectious Diseases

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

The content of rhesus macaque CD8+ T cells before and after isolation correspond to 55.7% and 93.9%, respectively.

Figure 1. EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit

In this example, starting with rhesus macaque PBMCs, the CD8+ T cell content (within the CD45+CD3+ gate) of start and final isolated fractions are 50.7% and 94.1%, respectively.

STEMCELL TECHNOLOGIES INC.'S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.