EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit

25-Minute cell isolation kit using immunomagnetic negative selection

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From: 798 USD

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25-Minute cell isolation kit using immunomagnetic negative selection
From: 798 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This kit has been verified for use with rhesus and cynomolgus macaques.
Advantages:
⦁ Fast, easy to use, and column-free
⦁ Up to 85% purity with high recovery
⦁ Untouched, viable cells
Components:
  • EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit (Catalog #19582)
    • EasySep™ Non-Human Primate CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ D Magnetic Particles, 2 x 1 mL
  • RoboSep™ Non-Human Primate CD4+ T Cell Isolation Kit (Catalog #19582RF)
    • EasySep™ Non-Human Primate CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ D Magnetic Particles, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells, CD4+
Species:
Non-Human Primate
Sample Source:
PBMC; Peripheral Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
HIV; Immunology; Infectious Diseases

Scientific Resources

Product Documentation

Educational Materials

(5)
Brochure
EasySep™: Isolate Cells from Non-Human Primates
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
Video
1:57
Fast and Easy Cell Isolation with EasySep™

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
T Cell Research
Cell Sourcing
Cell Isolation
Cell Activation and Expansion
Cell Characterization

Data and Publications

Data

The content of rhesus macaque CD4+ T cells before and after isolation correspond to 55.7% and 93.9%, respectively.

Figure 1. EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit

In this example, starting with rhesus macaque PBMCs, the CD4+ T cell content (within the CD45+CD3+ gate) of start and final isolated fractions are 49.5% and 92.3%, respectively.

STEMCELL TECHNOLOGIES INC.'S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.