EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit

Immunomagnetic negative selection of non-human primate CD4+ T cells from PBMCs, including rhesus and cynomolgus macaques

EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit

Immunomagnetic negative selection of non-human primate CD4+ T cells from PBMCs, including rhesus and cynomolgus macaques

From: 988 USD
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Immunomagnetic negative selection of non-human primate CD4+ T cells from PBMCs, including rhesus and cynomolgus macaques
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Product Advantages


  • Fast, easy to use, and column-free

  • Up to 85% purity with high recovery

  • Untouched, viable cells

What's Included

  • EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit (Catalog #19582)
    • EasySep™ Non-Human Primate CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ D Magnetic Particles, 2 x 1 mL
  • RoboSep™ Non-Human Primate CD4+ T Cell Isolation Kit (Catalog #19582RF)
    • EasySep™ Non-Human Primate CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ D Magnetic Particles, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

Easily and efficiently isolate highly purified CD4+ T cells from fresh or previously frozen non-human primate peripheral blood mononuclear cells (PBMCs) by immunomagnetic negative selection, with the EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD8+ T cell subsets, B cells, NK cells, monocytes, granulocytes, mast cells, dendritic cells, hematopoietic progenitors, and platelets. The magnetically labeled cells are then separated from the untouched desired CD4+ T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 25 minutes, the desired CD4+ T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This kit has been verified for use with rhesus and cynomolgus macaques.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD4+
Species
Non-Human Primate
Sample Source
PBMC, Peripheral Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
HIV, Immunology, Infectious Diseases

Data Figures

The content of rhesus macaque CD4+ T cells before and after isolation correspond to 55.7% and 93.9%, respectively.

Figure 1. EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit

In this example, starting with rhesus macaque PBMCs, the CD4+ T cell content (within the CD45+CD3+ gate) of start and final isolated fractions are 49.5% and 92.3%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
19582RF
Lot #
All
Language
English
Catalog #
19582
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
19582RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
19582RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
19582RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
19582
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
19582
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.