EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit
25-Minute cell isolation kit using immunomagnetic negative selection
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EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit -
RoboSep™ Non-Human Primate CD4+ T Cell Isolation Kit -
Label for EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
This kit has been verified for use with rhesus and cynomolgus macaques.
This kit has been verified for use with rhesus and cynomolgus macaques.
Advantages:
⦁ Fast, easy to use, and column-free
⦁ Up to 85% purity with high recovery
⦁ Untouched, viable cells
⦁ Up to 85% purity with high recovery
⦁ Untouched, viable cells
Components:
- EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit (Catalog #19582)
- EasySep™ Non-Human Primate CD4+ T Cell Isolation Cocktail, 1 mL
- EasySep™ D Magnetic Particles, 2 x 1 mL
- RoboSep™ Non-Human Primate CD4+ T Cell Isolation Kit (Catalog #19582RF)
- EasySep™ Non-Human Primate CD4+ T Cell Isolation Cocktail, 1 mL
- EasySep™ D Magnetic Particles, 2 x 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells, CD4+
Species:
Non-Human Primate
Sample Source:
PBMC; Peripheral Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
HIV; Immunology; Infectious Diseases
Related Products
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EasySep™ Non-Human Primate Custom Positive Selection Kit
Immunomagnetic positive selection kit -
EasySep™ Non-Human Primate Custom Enrichment Kit
Immunomagnetic negative selection kit
Labeling Antibodies
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Anti-Human CD4 Antibody, Clone OKT4
Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD4 -
Anti-Human CD8a Antibody, Clone RPA-T8
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
19582, 19582RF
All
Educational Materials
(5)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit
In this example, starting with rhesus macaque PBMCs, the CD4+ T cell content (within the CD45+CD3+ gate) of start and final isolated fractions are 49.5% and 92.3%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.