EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit

25-Minute cell isolation kit using immunomagnetic negative selection

EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit

25-Minute cell isolation kit using immunomagnetic negative selection

From: 932 USD
Catalog #
19582_C
25-Minute cell isolation kit using immunomagnetic negative selection

Product Advantages


  • Fast, easy to use, and column-free

  • Up to 85% purity with high recovery

  • Untouched, viable cells

What's Included

  • EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit (Catalog #19582)
    • EasySep™ Non-Human Primate CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ D Magnetic Particles, 2 x 1 mL
  • RoboSep™ Non-Human Primate CD4+ T Cell Isolation Kit (Catalog #19582RF)
    • EasySep™ Non-Human Primate CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ D Magnetic Particles, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)

Overview

The EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This kit has been verified for use with rhesus and cynomolgus macaques.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD4+
Species
Non-Human Primate
Sample Source
PBMC, Peripheral Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
HIV, Immunology, Infectious Diseases

Data Figures

The content of rhesus macaque CD4+ T cells before and after isolation correspond to 55.7% and 93.9%, respectively.

Figure 1. EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit

In this example, starting with rhesus macaque PBMCs, the CD4+ T cell content (within the CD45+CD3+ gate) of start and final isolated fractions are 49.5% and 92.3%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
19582, 19582RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
19582, 19582RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
19582, 19582RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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