EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit
Immunomagnetic negative selection of non-human primate CD4+ T cells from PBMCs, including rhesus and cynomolgus macaques
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In-lab demonstration
- EasySep™ Magnet
Magnet for column-free immunomagnetic separation
- EasySep™ Buffer
Cell separation buffer for use with EasySep™ cell separation kits
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Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells
Overview
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD8+ T cell subsets, B cells, NK cells, monocytes, granulocytes, mast cells, dendritic cells, hematopoietic progenitors, and platelets. The magnetically labeled cells are then separated from the untouched desired CD4+ T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 25 minutes, the desired CD4+ T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
This kit has been verified for use with rhesus and cynomolgus macaques.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (5)
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Labeling Antibodies
- Anti-Human CD4 Antibody, Clone OKT4
Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD4
- Anti-Human CD8a Antibody, Clone RPA-T8
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a
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EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.