EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit

Immunomagnetic negative selection cell isolation kit

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Immunomagnetic negative selection cell isolation kit
From: 389 USD

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Overview

The EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit is designed for the depletion of single or multiple unwanted cell types labeled with biotinylated antibodies. Labeled cells from mouse splenocytes or other tissues are targeted for removal by Streptavidin RapidSpheres™ and separated without the use of columns using an EasySep™ magnet. Desired cells are untouched and poured off into a new tube.
Advantages:
• Fast and easy-to-use
• No columns required
• Untouched, viable cells
Components:
  • EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Catalog #19860)
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
    • Normal Rat Serum, 2 mL
    • RoboSep™ Empty Vial
  • RoboSep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Catalog #19860RF)
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
    • Normal Rat Serum, 2 mL
    • RoboSep™ Empty Vial
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Other; B Cells; Dendritic Cells; Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Mesenchymal Stem and Progenitor Cells; Monocytes; Myeloid Cells; NK Cells; Plasma Cells; T Cells; Marrow Stromal Cells; Mononuclear Cells; Macrophages
Species:
Mouse
Sample Source:
Other; Spleen
Selection Method:
Negative; Depletion
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(7)

Data and Publications

Data

Typical Mouse Streptavidin Rapidspheres™ CD4 (CD3+CD8-) Depletion Profile

Figure 1. Typical Mouse Streptavidin Rapidspheres™ CD4 (CD3+CD8-) Depletion Profile

Typical Mouse Streptavidin Rapidspheres™ CD8 (CD3+CD4-) Depletion Profile

Figure 2. Typical Mouse Streptavidin Rapidspheres™ CD8 (CD3+CD4-) Depletion Profile

Typical Mouse Streptavidin Rapidspheres™ CD19 (CD19+CD45+) Depletion Profile

Figure 3. Typical Mouse Streptavidin Rapidspheres™ CD19 (CD19+CD45+) Depletion Profile

Publications

(4)
Cancer cell 2019 aug

Mechanisms of Progression of Myeloid Preleukemia to Transformed Myeloid Leukemia in Children with Down Syndrome.

M. Labuhn et al.

Abstract

Myeloid leukemia in Down syndrome (ML-DS) clonally evolves from transient abnormal myelopoiesis (TAM), a preleukemic condition in DS newborns. To define mechanisms of leukemic transformation, we combined exome and targeted resequencing of 111 TAM and 141 ML-DS samples with functional analyses. TAM requires trisomy 21 and truncating mutations in GATA1; additional TAM variants are usually not pathogenic. By contrast, in ML-DS, clonal and subclonal variants are functionally required. We identified a recurrent and oncogenic hotspot gain-of-function mutation in myeloid cytokine receptor CSF2RB. By a multiplex CRISPR/Cas9 screen in an in vivo murine TAM model, we tested loss-of-function of 22 recurrently mutated ML-DS genes. Loss of 18 different genes produced leukemias that phenotypically, genetically, and transcriptionally mirrored ML-DS.
Nature 2018 FEB

Population snapshots predict early haematopoietic and erythroid hierarchies.

B. K. Tusi et al.

Abstract

The formation of red blood cells begins with the differentiation of multipotent haematopoietic progenitors. Reconstructing the steps of this differentiation represents a general challenge in stem-cell biology. Here we used single-cell transcriptomics, fate assays and a theory that allows the prediction of cell fates from population snapshots to demonstrate that mouse haematopoietic progenitors differentiate through a continuous, hierarchical structure into seven blood lineages. We uncovered coupling between the erythroid and the basophil or mast cell fates, a global haematopoietic response to erythroid stress and novel growth factor receptors that regulate erythropoiesis. We defined a flow cytometry sorting strategy to purify early stages of erythroid differentiation, completely isolating classically defined burst-forming and colony-forming progenitors. We also found that the cell cycle is progressively remodelled during erythroid development and during a sharp transcriptional switch that ends the colony-forming progenitor stage and activates terminal differentiation. Our work showcases the utility of linking transcriptomic data to predictive fate models, and provides insights into lineage development in vivo.
Nature communications 2017 DEC

Alternative activation generates IL-10 producing type 2 innate lymphoid cells.

C. R. Seehus et al.

Abstract

Type 2 innate lymphoid cells (ILC2) share cytokine and transcription factor expression with CD4+ Th2 cells, but functional diversity of the ILC2 lineage has yet to be fully explored. Here, we show induction of a molecularly distinct subset of activated lung ILC2, termed ILC210. These cells produce IL-10 and downregulate some pro-inflammatory genes. Signals that generate ILC210 are distinct from those that induce IL-13 production, and gene expression data indicate that an alternative activation pathway leads to the generation of ILC210. In vivo, IL-2 enhances ILC210 generation and is associated with decreased eosinophil recruitment to the lung. Unlike most activated ILC2, the ILC210 population contracts after cessation of stimulation in vivo, with maintenance of a subset that can be recalled by restimulation, analogous to T-cell effector cell and memory cell generation. These data demonstrate the generation of a previously unappreciated IL-10 producing ILC2 effector cell population.
Nature communications 2016

Th2 and eosinophil responses suppress inflammatory arthritis.

Chen Z et al.

Abstract

Th2-eosinophil immune responses are well known for mediating host defence against helminths. Herein we describe a function of Th2-eosinophil responses in counteracting the development of arthritis. In two independent models of arthritis, Nippostrongylus brasiliensis infection leads to Th2 and eosinophil accumulation in the joints associated with robust inhibition of arthritis and protection from bone loss. Mechanistically, this protective effect is dependent on IL-4/IL-13-induced STAT6 pathway. Furthermore, we show that eosinophils play a central role in the modulation of arthritis probably through the increase of anti-inflammatory macrophages into arthritic joints. The presence of these pathways in human disease is confirmed by detection of GATA3-positive cells and eosinophils in the joints of rheumatoid arthritis patients. Taken together, these results demonstrate that eosinophils and helminth-induced activation of the Th2 pathway axis effectively mitigate the course of inflammatory arthritis.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.