EasySep™ Mouse F4/80 Positive Selection Kit
Immunomagnetic positive selection of F4/80+ cells from mouse splenocytes, lung tissue, and peritoneal lavage
Request Pricing
Thank you for your interest in this product. Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
- EasySep™ Magnet
Magnet for column-free immunomagnetic separation
- EasySep™ Buffer
Cell separation buffer
-
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells
Overview
Using immunomagnetic positive selection, F4/80+ cells are labeled with antibodies and dextran-coated magnetic particles (RapidSpheres™), for column-free cell separation using an EasySep™ magnet. Isolated cells are highly purified and ready for your downstream applications.
Visit www.EasySep.com to learn more about column-free EasySep™ cell separation technology.
Data Figures
Figure 1. Typical Profile for F4/80+ Cells Isolated from Lung Tissue Using the EasySep™ Mouse F4/80 Positive Selection Kit
Starting with a naïve mouse lung single-cell suspension, the F4/80+ cell content of the isolated fraction is typically 94.3 ± 2.8% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 26.3% and 95.3%, respectively.
Figure 2. Typical Profile for F4/80+ Cells Isolated from Peritoneal Lavage Using the EasySep™ Mouse F4/80 Positive Selection Kit
Starting with lavage cells from the peritoneal cavity of a naïve mouse, the F4/80+ cell content of the isolated fraction is typically 97.0 ± 0.4% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 47.9% and 97.0%, respectively.
Figure 3. Typical Profile for F4/80+ Cells Isolated from Spleen Using the EasySep™ Mouse F4/80 Positive Selection Kit
Starting with naïve mouse splenocytes, the F4/80+ cell content of the isolated fraction is typically 88.8 ± 3.4% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 5.4% and 90.6%, respectively.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (7)
Related Products
- Mouse TNF-alpha ELISA Kit
For detection and measurement of mouse tumor necrosis factor-alpha
- EasySep™ Mouse CD11b Positive Selection Kit...
Immunomagnetic positive selection cell isolation kit
Labeling Antibodies
- Anti-Mouse CD11b Antibody, Clone M1/70
Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b
- Anti-Mouse F4/80 Antibody, Clone BM8
Rat monoclonal IgG2a antibody against human, mouse F4/80
- Anti-Mouse CD45 Antibody, Clone 30-F11
Rat monoclonal IgG2b antibody against mouse CD45
Item added to your cart
EasySep™ Mouse F4/80 Positive Selection Kit
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.