EasySep™ Mouse F4/80 Positive Selection Kit
Immunomagnetic positive selection of F4/80+ cells from mouse splenocytes, lung tissue, and peritoneal lavage
EasySep™ Mouse F4/80 Positive Selection Kit
Immunomagnetic positive selection of F4/80+ cells from mouse splenocytes, lung tissue, and peritoneal lavage
Product Advantages
- Fast and easy-to-use
- Up to 90% purity
- No columns required
- Isolated cells are not fluorochrome-labeled
What's Included
- EasySep™ Mouse F4/80 Positive Selection Component A, 0.3 mL
- EasySep™ Mouse F4/80 Positive Selection Component B, 0.6 mL
- EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
- Normal Rat Serum, 2 mL
Overview
Using immunomagnetic positive selection, F4/80+ cells are labeled with antibodies and dextran-coated magnetic particles (RapidSpheres™), for column-free cell separation using an EasySep™ magnet. Isolated cells are highly purified and ready for your downstream applications.
Visit www.EasySep.com to learn more about column-free EasySep™ cell separation technology.
Data Figures
Figure 1. Typical Profile for F4/80+ Cells Isolated from Lung Tissue Using the EasySep™ Mouse F4/80 Positive Selection Kit
Starting with a naïve mouse lung single-cell suspension, the F4/80+ cell content of the isolated fraction is typically 94.3 ± 2.8% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 26.3% and 95.3%, respectively.
Figure 2. Typical Profile for F4/80+ Cells Isolated from Peritoneal Lavage Using the EasySep™ Mouse F4/80 Positive Selection Kit
Starting with lavage cells from the peritoneal cavity of a naïve mouse, the F4/80+ cell content of the isolated fraction is typically 94.4 ± 2.9% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 40.9% and 94.1%, respectively.
Figure 3. Typical Profile for F4/80+ Cells Isolated from Spleen Using the EasySep™ Mouse F4/80 Positive Selection Kit
Starting with naïve mouse splenocytes, the F4/80+ cell content of the isolated fraction is typically 88.8 ± 3.4% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 5.4% and 90.6%, respectively.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (7)
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
