EasySep™ Human Th17 Cell Enrichment Kit II

Immunomagnetic cell isolation kit using particle release technology
EasySep™ Human Th17 Cell Enrichment Kit II

Immunomagnetic positive selection of human Th17 cells

2 x 10^9 cells
Catalog # 17862
1,993 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human Th17 Enrichment Kit II is a two-step method designed to enrich for human Th17 cells from fresh peripheral blood mononuclear cells. First, CCR6+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the cells and unwanted non-CD4+ T cells, CD45RA+ cells, and CXCR3+ cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction is enriched for IL-17-producing Th17 cells.

This product can be used in place of the EasySep™ Human Th17 Cell Enrichment Kit (Catalog #18162) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 98% purity
• No columns required
Components
  • EasySep™ Human Th17 Enrichment Kit II (Catalog #17862)
    • EasySep™ Human CCR6 Positive Selection Cocktail II, 0.5 mL
    • EasySep™ Releasable RapidSpheres™, 4 x 1 mL
    • EasySep™ Human CD4+CXCR3- T Cell Pre-Enrichment Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • EasySep™ Release Buffer, 2 x 1 mL
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Human Th17 Cell Enrichment Kit II
Catalog #
17862
Lot #
All
Language
English

Educational Materials (7)

Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Human Immune Cytokines
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data


Starting with fresh PBMCs, the Th17 cell content (CD4+CXCR3-CCR6+) of the isolated fraction typically ranges from 96 - 98%. Following overnight stimulation with PMA-Ionomycin, 6 - 19% of the isolated cells are IL-17+ by intracellular flow cytometry. These values vary widely among donors. IFN-γ-producing cells are typically < 5% of the enriched fraction.

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