EasySep™ Human Resting CD4+ T Cell Isolation Kit

Immunomagnetic negative selection kit

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From: 935 USD

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Immunomagnetic negative selection kit
From: 935 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human Resting CD4+ T Cell Isolation Kit is designed to isolate resting CD4+ T cells from fresh or previously frozen human peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages:
• Fast, easy-to-use and column-free
• Up to 99% purity
• Isolated cells are untouched
Components:
  • EasySep™ Human Resting CD4+ T Cell Isolation Kit (Catalog #17962)
    • EasySep™ Human Resting CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ Human CD25 Depletion Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • RoboSep™ Empty Vial
  • RoboSep™ Human Resting CD4+ T Cell Isolation Kit (Catalog #17962RF)
    • EasySep™ Human Resting CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ Human CD25 Depletion Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • RoboSep™ Empty Vial
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells, CD4+; T Cells
Species:
Human
Sample Source:
PBMC; Leukapheresis
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(7)
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
The Immune Response to HIV Poster
Wallchart
Human Immune Cytokines
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Video
1:23
How to Isolate Cells with EasySep™

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
T Cell Research
Cell Sourcing
Cell Isolation
Cell Activation and Expansion
Cell Characterization

Data and Publications

Data

(A) Starting with fresh PBMCs, the resting CD4+ T cell content (CD3+CD4+CD8-CD25-CD69-HLA-DR-) of the isolated fraction is typically 95.5 ± 6.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 35% and 99%, respectively. (B) Starting with fresh PBMCs that have elevated CD25/CD69/HLA-DR expression, the resting CD4+ T cell content of the isolated fraction is typically 88.0 ± 7.0% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 8% and 85%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.