EasySep™ Human Pan-ILC Enrichment Kit

Immunomagnetic negative selection cell enrichment kit
Catalog #
17975_C
Immunomagnetic negative selection cell enrichment kit
From: 848 USD
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Required Products
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  2. "The Big Easy" EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

The EasySep™ Human Pan-ILC Enrichment Kit is designed to enrich Group 1, 2, and 3 Innate Lymphoid Cells (ILC1, 2, and 3) from leukapheresis products by immunomagnetic negative selection. Unwanted cells are targeted for removal by Tetrameric Antibody Complexes directed against non-ILCs and dextran-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as enriched ILCs are simply poured off into a new tube. Isolated cells are immediately available for flow sorting and other downstream applications.
Advantages
• Fast, easy-to-use and column-free
• Untouched, viable cells
• Facilitates rapid flow sorting of ILCs
Components
  • EasySep™ Human Pan-ILC Enrichment Kit (Catalog #17975)
    • EasySep™ Human Pan-ILC Enrichment Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
  • RoboSep™ Human Pan-ILC Enrichment Kit (Catalog #17975RF)
    • EasySep™ Human Pan-ILC Enrichment Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Pan-ILC Enrichment Kit
Catalog #
17975
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Pan-ILC Enrichment Kit
Catalog #
17975RF
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
EasySep™ Human Pan-ILC Enrichment Kit
Catalog #
17975
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
RoboSep™ Human Pan-ILC Enrichment Kit
Catalog #
17975RF
Lot #
All
Language
English

Educational Materials (13)

Brochure
Cell Enrichment Prior to Cell Sorting
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Technical Bulletin
Gating Strategy for the Identification of ILC2s
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Innate Lymphoid Cells
Wallchart
Human Immune Cytokines
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Video
How to Pipet Off Samples During Cell Isolation Using the EasyEights™ EasySep™ Cell Separation Magnet
1:16
How to Pipet Off Samples During Cell Isolation Using the EasyEights™ EasySep™ Cell Separation Magnet
Scientific Poster
Pre-enrichment of Human ILCs Improves Sorting Efficiency While Maintaining Functionality
Scientific Poster
Efficient Enrichment of Functional ILC Subsets from Human PBMCs by Immunomagnetic Selection
Load More Educational Materials

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Typical EasySep™ Human Pan-ILC Enrichment Profile

Figure 1. Typical EasySep™ Human Pan-ILC Enrichment Profile

Starting with fresh leukapheresis samples, the total ILC content (Lin-CD45+CD127+) of the enriched fraction typically ranges from 17 - 85%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.09% and 45% (or 0.1% and 54% of CD45+ cells), respectively.

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