EasySep™ Human IgG+ Memory B Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology
EasySep™ Human IgG+ Memory B Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

Catalog # 17868
905 USD
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human IgG+ Memory B Cell Isolation Kit isolates IgG+CD27+ B cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD27+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD27+ cells, and unwanted non-B cells, as well as IgM+, IgD+ and IgA+ cells are depleted using EasySep™ Dextran RapidSpheres™. The final isolated fraction contains IgG+CD27+ memory B cells, which readily secrete IgG in culture supernatants in the presence of appropriate stimuli.
Advantages
• Highly purified IgG+ memory B cells isolated in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
• Optional isolation of untouched naïve B cells from the same sample
Components
  • EasySep™ Human IgG+ Memory B Cell Isolation Kit (Catalog #17868)
    • EasySep™ Human CD27 Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 2 x 1 mL
    • EasySep™ Release Buffer, 2 x 1 mL
    • EasySep™ Human Memory B Cell Pre-Enrichment Cocktail, 2 x 1 mL
    • EasySep™ Human IgM/IgD/IgA Depletion Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human IgG+ Memory B Cell Isolation Kit
Catalog #
17868
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human IgG+ Memory B Cell Isolation Kit
Catalog #
17868
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human IgG+ Memory B Cell Isolation Kit
Catalog #
17868
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human IgG+ Memory B Cell Isolation Kit
Catalog #
17868
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Human IgG+ Memory B Cell Isolation Kit
Catalog #
17868
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Human IgG+ Memory B Cell Isolation Kit
Catalog #
17868
Lot #
All
Language
English

Educational Materials (9)

Brochure
Human B Cell Isolation Product Selection
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Antigen Processing and Presentation
Wallchart
Human Immune Cytokines
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

FACS Profile Results with EasySep™ Human IgG+ Memory B Cell Isolation Kit

Figure 1. EasySep™ Human IgG+ Memory B Cell Isolation Kit

Starting with PBMCs, the IgG+ memory B cell content (CD19+CD27+IgG+) of the isolated fraction is typically 82.6 ± 7.0% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 1.0% and 90.2% respectively.

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