EasySep™ Human HLA-DR Positive Selection and Depletion Kit

Immunomagnetic positive selection and cell depletion kit

EasySep™ Human HLA-DR Positive Selection and Depletion Kit

Immunomagnetic positive selection and cell depletion kit

From: 725 USD
Catalog #
100-0980_C
Immunomagnetic positive selection and cell depletion kit

Product Advantages

• Fast and easy-to-use
• Up to 98% purity
• No columns required

What's Included

  • EasySep™ Human HLA-DR Positive Selection and Depletion Kit (Catalog #100-0980)
    • EasySep™ Human HLA-DR Positive Selection and Depletion Cocktail, 1 x 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL

Overview

Efficiently isolate or deplete HLA-DR+ cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples in as little as 25 minutes; and immediately use the desired cells in downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Use the EasySep™ Human HLA-DR Positive Selection and Depletion Kit to label HLA-DR+ cells with antibodies and magnetic particles. The cells are then subsequently separated using an EasySep™ magnet—without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off. Depending on your research needs, either fraction may be used downstream.

This kit targets HLA-DR, a human major histocompatibility complex (MHC) class II receptor expressed on the cell surface of antigen-presenting cells (APCs), including dendritic cells, B cells, monocytes, macrophages, and activated T cells (Saraiva et al. Front Immunol, 2018). Research on APCs is relevant to the study of autoimmune diseases, viral infections, and cancer, to mention a few examples.

Learn more about how immunomagnetic EasySep™ technology works.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• RoboSep™-S (Catalog #21000)
Cell Type
B Cells, Dendritic Cells, Macrophages, Monocytes
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Depletion, Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Cancer, Immunology, Infectious Diseases, Transplantation Research

Data Figures

Figure 1. Using the EasySep™ Human HLA-DR Positive Selection and Depletion Kit with washed or lysed leukopheresis samples, the frequencies of HLA-DR+ cells in the start, final isolated, and final depleted fractions are 50.7%, 96.0%, and 1.0%, respectively.

Figure 1. Typical EasySep™ Human HLA-DR Positive Selection Profile

Starting with washed or lysed leukopheresis samples, the HLA-DR cell content of the isolated fraction is typically 91.9 ± 6.4% after positive selection and 2.0 ± 1.8% after depletion (mean ± SD using the EasySep™ Magnet). In the above example, the frequencies of HLA-DR+ cells in the start, final isolated, and final depleted fractions are 50.7%, 96.0%, and 1.0%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0980
Lot #
All
Language
English
Catalog #
100-0982
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0980
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0982
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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