Easily isolate or deplete human HLA-DR+ cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples, using immunomagnetic positive selection, with the EasySep™ Human HLA-DR Positive Selection and Depletion Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, cells of interest are labeled with antibody complexes recognizing HLA-DR and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring off the unlabeled cells. The HLA-DR+ cells remain in the tube. Following magnetic cell isolation in as little as 25 minutes, desired cells from either fraction are ready for downstream applications, such as flow cytometry, culture, or DNA/RNA extraction. This kit targets HLA-DR, a human major histocompatibility complex (MHC) class II receptor expressed on the cell surface of antigen-presenting cells (APCs), including dendritic cells, B cells, monocytes, macrophages, and activated T cells (Saraiva et al. Front Immunol, 2018). Research on APCs is relevant to the study of autoimmune diseases, viral infections, cancer, and more.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. STEMCELL Technologies offers a wide selection of antibodies for custom separation and will work with you to isolate a cell type of interest. Please contact our technical support team at techsupport@stemcell.com. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.