EasySep™ Human HLA-DR Positive Selection and Depletion Kit
Immunomagnetic positive selection or depletion of human HLA-DR+ cells from fresh or previously frozen human PBMCs or leukapheresis samples
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Overview
In this EasySep™ positive selection procedure, cells of interest are labeled with antibody complexes recognizing HLA-DR and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring off the unlabeled cells. The HLA-DR+ cells remain in the tube. Following magnetic cell isolation in as little as 25 minutes, desired cells from either fraction are ready for downstream applications, such as flow cytometry, culture, or DNA/RNA extraction. This kit targets HLA-DR, a human major histocompatibility complex (MHC) class II receptor expressed on the cell surface of antigen-presenting cells (APCs), including dendritic cells, B cells, monocytes, macrophages, and activated T cells (Saraiva et al. Front Immunol, 2018). Research on APCs is relevant to the study of autoimmune diseases, viral infections, cancer, and more.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. STEMCELL Technologies offers a wide selection of antibodies for custom separation and will work with you to isolate a cell type of interest. Please contact our technical support team at techsupport@stemcell.com. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Data Figures

Figure 1. Typical EasySep™ Human HLA-DR Positive Selection Profile
Starting with washed or lysed leukopheresis samples, the HLA-DR cell content of the isolated fraction is typically 91.9 ± 6.4% after positive selection and 2.0 ± 1.8% after depletion (mean ± SD using the EasySep™ Magnet). In the above example, the frequencies of HLA-DR+ cells in the start, final isolated, and final depleted fractions are 50.7%, 96.0%, and 1.0%, respectively.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
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EasySep™ Human HLA-DR Positive Selection and Depletion Kit
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.