Efficiently isolate or deplete HLA-DR+ cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples in as little as 25 minutes; and immediately use the desired cells in downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Use the EasySep™ Human HLA-DR Positive Selection and Depletion Kit to label HLA-DR+ cells with antibodies and magnetic particles. The cells are then subsequently separated using an EasySep™ magnet—without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off. Depending on your research needs, either fraction may be used downstream.

This kit targets HLA-DR, a human major histocompatibility complex (MHC) class II receptor expressed on the cell surface of antigen-presenting cells (APCs), including dendritic cells, B cells, monocytes, macrophages, and activated T cells (Saraiva et al. Front Immunol, 2018). Research on APCs is relevant to the study of autoimmune diseases, viral infections, and cancer, to mention a few examples.

Learn more about how immunomagnetic EasySep™ technology works.