EasySep™ Human HLA-DR Positive Selection and Depletion Kit

Immunomagnetic positive selection or depletion of human HLA-DR+ cells from fresh or previously frozen human PBMCs or leukapheresis samples

EasySep™ Human HLA-DR Positive Selection and Depletion Kit

Immunomagnetic positive selection or depletion of human HLA-DR+ cells from fresh or previously frozen human PBMCs or leukapheresis samples

From: 745 USD
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Immunomagnetic positive selection or depletion of human HLA-DR+ cells from fresh or previously frozen human PBMCs or leukapheresis samples
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Product Advantages

• Fast and easy-to-use
• Up to 98% purity
• No columns required

What's Included

  • EasySep™ Human HLA-DR Positive Selection and Depletion Kit (Catalog #100-0980)
    • EasySep™ Human HLA-DR Positive Selection and Depletion Cocktail, 1 x 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL

Overview

Easily isolate or deplete human HLA-DR+ cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples, using immunomagnetic positive selection, with the EasySep™ Human HLA-DR Positive Selection and Depletion Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, cells of interest are labeled with antibody complexes recognizing HLA-DR and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring off the unlabeled cells. The HLA-DR+ cells remain in the tube. Following magnetic cell isolation in as little as 25 minutes, desired cells from either fraction are ready for downstream applications, such as flow cytometry, culture, or DNA/RNA extraction. This kit targets HLA-DR, a human major histocompatibility complex (MHC) class II receptor expressed on the cell surface of antigen-presenting cells (APCs), including dendritic cells, B cells, monocytes, macrophages, and activated T cells (Saraiva et al. Front Immunol, 2018). Research on APCs is relevant to the study of autoimmune diseases, viral infections, cancer, and more.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. STEMCELL Technologies offers a wide selection of antibodies for custom separation and will work with you to isolate a cell type of interest. Please contact our technical support team at techsupport@stemcell.com. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• RoboSep™-S (Catalog #21000)
Cell Type
B Cells, Dendritic Cells, Macrophages, Monocytes
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Depletion, Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Cancer, Immunology, Infectious Diseases, Transplantation Research

Data Figures

Figure 1. Using the EasySep™ Human HLA-DR Positive Selection and Depletion Kit with washed or lysed leukopheresis samples, the frequencies of HLA-DR+ cells in the start, final isolated, and final depleted fractions are 50.7%, 96.0%, and 1.0%, respectively.

Figure 1. Typical EasySep™ Human HLA-DR Positive Selection Profile

Starting with washed or lysed leukopheresis samples, the HLA-DR cell content of the isolated fraction is typically 91.9 ± 6.4% after positive selection and 2.0 ± 1.8% after depletion (mean ± SD using the EasySep™ Magnet). In the above example, the frequencies of HLA-DR+ cells in the start, final isolated, and final depleted fractions are 50.7%, 96.0%, and 1.0%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0982
Lot #
All
Language
English
Catalog #
100-0980
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0982
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0982
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0980
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0980
Lot #
All
Language
English