EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit

Fast & easy isolation of human extracellular vesicles using immunomagnetic positive selection

EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit

Fast & easy isolation of human extracellular vesicles using immunomagnetic positive selection

EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit
20 mL of biofluid
439 USD
Catalog # 17895

Fast & easy isolation of human extracellular vesicles using immunomagnetic positive selection

Product Advantages


  • Fast, easy to use, and column-free.

  • Avoid the need for ultracentrifugation and associated time consuming EV isolation methods.

  • Obtain highly purified EVs without contaminating biofluid components.

What's Included

  • EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit (Catalog #17895)
    • EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Cocktail 1 x 1 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 2 x 1 mL
    • NOTE: Isolated extracellular vesicles should not be released from the EasySep™ Releasable Rapidspheres™ 50201

Overview

The EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit isolates highly purified human extracellular vesicles from plasma/serum and cell culture conditioned medium by immunomagnetic positive selection. Extracellular vesicles are labeled with Tetrameric Antibody Complexes recognizing CD63 and magnetic particles. Labeled extracellular vesicles are separated without columns using an EasySep™ magnet. Unwanted biofluid components are simply poured off, while desired extracellular vesicles remain in the tube. Isolated extracellular vesicles are available for downstream applications such as DNA/RNA extraction, Western blot, or mass spectometry.​
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype
Cell Isolation Kits
Cell Type
Other
Sample Source
Other
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Extracellular Vesicle Research

Data Figures

Postive isolation of EVs with the CD63 tetraspanin marker from human plasma and MSC-conditioned medium.

Figure 1. Typical Western Blot Analyses of EVs Isolated from Human Plasma and Mesenchymal Stromal Cell (MSC)-Conditioned Medium

The Western blot analyses in the above examples show positive isolation of EVs with the CD63 tetraspanin marker from (A) human plasma and (B) MSC-conditioned medium. Western blots were run under non-reducing conditions.

Figure 2. Tetraspanin Protein Expression and Recovery of EVs Using EasySep™ Human Pan/CD9/CD63/CD81 Extracellular Vesicle Positive Selection Kits

EVs were isolated from plasma using either EasySep™ Human Extracellular Vesicle Positive Selection Kits or differential ultracentrifugation (2 x 70 min, 100,000 xg) and isolated fractions were analyzed by western blot for tetraspanin protein expression.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
17895
Lot #
All
Language
English
Catalog #
17895
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17895
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17895
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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