EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit

Fast & easy isolation of human extracellular vesicles using immunomagnetic positive selection
EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit

Fast & easy isolation of human extracellular vesicles using immunomagnetic positive selection

20 mL of biofluid
Catalog # 17895
395 USD
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

The EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit isolates highly purified human extracellular vesicles from plasma/serum and cell culture conditioned medium by immunomagnetic positive selection. Extracellular vesicles are labeled with Tetrameric Antibody Complexes recognizing CD63 and magnetic particles. Labeled extracellular vesicles are separated without columns using an EasySep™ magnet. Unwanted biofluid components are simply poured off, while desired extracellular vesicles remain in the tube. Isolated extracellular vesicles are available for downstream applications such as DNA/RNA extraction, Western blot, or mass spectometry.​
Advantages
⦁ Fast, easy to use, and column-free.
⦁ Avoid the need for ultracentrifugation and associated time consuming EV isolation methods.
⦁ Obtain highly purified EVs without contaminating biofluid components.
Components
  • EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit (Catalog #17895)
    • EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Cocktail 1 x 1 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 2 x 1 mL
    • NOTE: Isolated extracellular vesicles should not be released from the EasySep™ Releasable Rapidspheres™ 50201
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype
Cell Isolation Kits
Cell Type
Other
Sample Source
Other
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Extracellular Vesicle Research

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit
Catalog #
17895
Lot #
All
Language
English
Document Type
Special Protocol
Product Name
EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit
Catalog #
17895
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit
Catalog #
17895
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit
Catalog #
17895
Lot #
All
Language
English

Educational Materials(3)

Brochure
Isolate and Purify Extracellular Vesicles
Wallchart
Mesenchymal Stromal Cell-Derived Exosomes: Biogenesis and Cargoes
Webinar
35:31
Decoding Intercellular Communication via Hepatocyte-Secreted Extracellular Vesicles

Data and Publications

Data

Postive isolation of EVs with the CD63 tetraspanin marker from human plasma and MSC-conditioned medium.

Figure 1. Typical Western Blot Analyses of EVs Isolated from Human Plasma and Mesenchymal Stromal Cell (MSC)-Conditioned Medium

The Western blot analyses in the above examples show positive isolation of EVs with the CD63 tetraspanin marker from (A) human plasma and (B) MSC-conditioned medium. Western blots were run under non-reducing conditions.

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