EasySep™ Human Eosinophil Isolation Kit

11-Minute cell isolation kit using immunomagnetic negative selection
Catalog #
17956_C
11-Minute cell isolation kit using immunomagnetic negative selection
From: 621 EUR
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human Eosinophil Isolation Kit is designed to isolate eosinophils from a polymorphonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-eosinophils and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

The kit is compatible with cells prepared using either Lymphoprep™ (Catalog #07801) centrifugation (preferred for higher cell purities) or HetaSep™ (Catalog #07906) sedimentation (preferred for faster, lysis-free sample processing).

This product replaces the EasySep™ Human Eosinophil Enrichment Kit (Catalog #19256) for even faster cell isolations.
Advantages
• Fast, easy-to-use and column-free
• Up to 99% purity
• Untouched, viable cells
Components
  • EasySep™ Human Eosinophil Isolation Kit (Catalog #17956)
    • EasySep™ Human Eosinophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
  • RoboSep™ Human Eosinophil Isolation Kit with Filter Tips (Catalog #17956RF)
    • EasySep™ Human Eosinophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets
Species
Human
Sample Source
PMNC, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Eosinophil Isolation Kit
Catalog #
17956
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Eosinophil Isolation Kit
Catalog #
17956RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Eosinophil Isolation Kit
Catalog #
17956
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Eosinophil Isolation Kit
Catalog #
17956
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Eosinophil Isolation Kit
Catalog #
17956
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Eosinophil Isolation Kit
Catalog #
17956RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Eosinophil Isolation Kit
Catalog #
17956RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Eosinophil Isolation Kit
Catalog #
17956RF
Lot #
All
Language
English

Educational Materials (8)

Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent
2:10
How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Typical EasySep™ Human Eosinophil Isolation Profile

Starting with whole blood prepared using RBC lysis or HetaSep™, the eosinophil content (CD16-CD66b+CD45+) of the isolated fraction is typically 96.5 ± 2.5% (mean ± SD using the purple EasySep™ Magnet). In the above examples, the purities of the start and final isolated fractions prepared using RBC lysis are 1.8% and 97.8% (gated on CD45+), respectively, and when prepared using HetaSep™, the purities are 2.4% and 96.8% (gated on CD45+), respectively.

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