Overview
• Up to 87.4% purity
• Isolated cells are untouched
- EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232)
- EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
- EasySep™ Dextran Selection Cocktail, 1 mL
- EasySep™ Magnetic Particles, 3 x 1 mL
- RoboSep™ CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232RF)
- EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
- EasySep™ Dextran Selection Cocktail, 1 mL
- EasySep™ Magnetic Particles, 3 x 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Scientific Resources
Product Documentation
Educational Materials
(9)Frequently Asked Questions
Can EasySep™ be used for either positive or negative selection?
How does the separation work?
Which columns do I use?
How can I analyze the purity of my enriched sample?
Can EasySep™ separations be automated?
Can EasySep™ be used to isolate rare cells?
Are the EasySep™ magnetic particles FACS-compatible?
Can the EasySep™ magnetic particles be removed after enrichment?
Can I alter the separation time in the magnet?
For positive selection, can I perform more than 3 separations to increase purity?
How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Figure 1. Typical EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Profile
Starting with fresh PBMCs, the CD3+CD4+CD127lowCD49d-CD25+FOXP3+ cell content of the enriched fraction typically ranges from 57.4 - 87.4%. Donor to donor variability is unavoidable and may affect the purity of the enriched fraction. In the example above, the purities of the start and enriched fractions are 1.84% and 84.1%, respectively.