EasySep™ Human Basophil Isolation Kit

13-Minute cell isolation kit using immunomagnetic negative selection

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From: 740 USD

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13-Minute cell isolation kit using immunomagnetic negative selection
From: 740 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human Basophil Isolation Kit is designed to isolate basophils from a polymorphonuclear cell-rich fraction by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-basophils and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation of fresh peripheral blood.

This product replaces the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) for even faster cell isolations.
Advantages:
• Fast, easy-to-use and column-free
• Up to 98% purity
• Isolated cells are untouched
Components:
  • EasySep™ Human Basophil Isolation Kit (Catalog #17969)
    • EasySep™ Human Basophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
  • RoboSep™ Human Basophil Isolation Kit with Filter Tips (Catalog #17969RF)
    • EasySep™ Human Basophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Granulocytes and Subsets
Species:
Human
Sample Source:
PMNC; Whole Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(8)
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
1:37
How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
Video
2:10
HetaSep™: A Guide to Isolating Nucleated Cells from Whole Blood

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Granulocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization

Data and Publications

Data

Figure 1. Typical EasySep™ Human Basophil Isolation Profile

Starting with HetaSep™-processsed human peripheral blood, the basophil content (CD45+CD123+IgE+) of the isolated fraction is typically 94.0 ± 2.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 0.86% and 95.6% (gated on CD45+), respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.