EasySep™ Human Basophil Isolation Kit
13-Minute cell isolation kit using immunomagnetic negative selection
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EasySep™ Human Basophil Isolation Kit -
EasySep™ Human Basophil Isolation Kit -
Label for EasySep™ Human Basophil Isolation Kit
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Required Products
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For depletion of red blood cells from fresh blood samples and isolation of nucleated cells -
Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Human Basophil Isolation Kit is designed to isolate basophils from a polymorphonuclear cell-rich fraction by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-basophils and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation of fresh peripheral blood.
This product replaces the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) for even faster cell isolations.
The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation of fresh peripheral blood.
This product replaces the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) for even faster cell isolations.
Advantages:
• Fast, easy-to-use and column-free
• Up to 98% purity
• Isolated cells are untouched
• Up to 98% purity
• Isolated cells are untouched
Components:
- EasySep™ Human Basophil Isolation Kit (Catalog #17969)
- EasySep™ Human Basophil Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 2 x 1 mL
- RoboSep™ Human Basophil Isolation Kit with Filter Tips (Catalog #17969RF)
- EasySep™ Human Basophil Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 2 x 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Granulocytes and Subsets
Species:
Human
Sample Source:
PMNC; Whole Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology
Related Products
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EasySep™ Human Pan-Granulocyte Isolation Kit
Immunomagnetic negative selection cell isolation kit
Labeling Antibodies
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Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6
Mouse monoclonal IgG1 antibody against human, rhesus, sooty mangabey CD123 (IL-3Rα) -
Anti-Human CD45 Antibody, Clone HI30
Mouse monoclonal IgG1 antibody against human, chimpanzee CD45
Scientific Resources
Product Documentation
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(8)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. Typical EasySep™ Human Basophil Isolation Profile
Starting with HetaSep™-processsed human peripheral blood, the basophil content (CD45+CD123+IgE+) of the isolated fraction is typically 94.0 ± 2.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 0.86% and 95.6% (gated on CD45+), respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.