EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit

EasySep™ HLA Whole Blood B Cell Positive Selection Kit

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EasySep™ HLA Whole Blood B Cell Positive Selection Kit
From: 538 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit is designed to isolate B cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19, CD20 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors, but is lost upon maturation to plasma cells. The antigen CD20 is expressed on all stages of B cell development except on pro B cells or plasma cells.

This product replaces the EasySep™ HLA Whole Blood B Cell Positive Selection Kit (Catalog #18184HLA) for even faster cell isolations.
Advantages:
• –Fast and easy-to-use
• Up to 97% purity
• No columns required
Components:
  • EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit (Catalog #17886)
    • EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit (Catalog #17886RF)
    • EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Subtype:
Cell Isolation Kits
Cell Type:
B Cells
Species:
Human
Sample Source:
Buffy Coat; Peripheral Blood
Selection Method:
Positive
Brand:
EasySep; RoboSep
Area of Interest:
Chimerism; HLA; Immunology

Scientific Resources

Product Documentation

Educational Materials

(11)
Brochure
HLA Antibodies for Purity Assessment
Brochure
EasySep™ Cell Separation Technology
Brochure
Cell Separation Products for HLA Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
1:41
RoboSep™-S Introduction
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Webinar
56:02
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
B Cell Research
Cell Sourcing
Cell Isolation
Cell Characterization
Chimerism Analysis
Cell Isolation
Characterization

Data and Publications

Data

Starting with human whole blood, the B cell content (CD19+CD20+) of the isolated fraction is typically 92.5 ± 5.6% (gated on CD45+ cells; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 4.5% and 92.2%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.