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EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit

EasySep™ HLA Whole Blood B Cell Positive Selection Kit

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit

EasySep™ HLA Whole Blood B Cell Positive Selection Kit

From: 628 USD
Catalog #
17886_C
EasySep™ HLA Whole Blood B Cell Positive Selection Kit

Product Advantages


  • Fast and easy-to-use

  • Up to 97% purity

  • No columns required

What's Included

  • EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit (Catalog #17886)
    • EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit (Catalog #17886RF)
    • EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Products for Your Protocol
To see all required products for your protocol, please consult the Product Information Sheet.
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  2. "The Big Easy" EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

The EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit is designed to isolate B cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19, CD20 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors, but is lost upon maturation to plasma cells. The antigen CD20 is expressed on all stages of B cell development except on pro B cells or plasma cells.

This product replaces the EasySep™ HLA Whole Blood B Cell Positive Selection Kit (Catalog #18184HLA) for even faster cell isolations.
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Buffy Coat, Peripheral Blood
Selection Method
Positive
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology

Data Figures

Starting with human whole blood, the B cell content (CD19+CD20+) of the isolated fraction is typically 92.5 ± 5.6% (gated on CD45+ cells; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 4.5% and 92.2%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet - Buffy Coat or Whole Blood
Product Name
EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886
Lot #
All
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886
Lot #
All
Language
English
Document Type
Product Information Sheet - Buffy Coat or Whole Blood
Product Name
RoboSep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886RF
Lot #
All
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
RoboSep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit
Catalog #
17886RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (13)

HLA Antibodies for Purity Assessment
Brochure
HLA Antibodies for Purity Assessment
Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Human B Cell Isolation Product Selection
Brochure
Human B Cell Isolation Product Selection
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
4:55
Video
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subse...

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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