• Up to 3 log depletion of normal hematopoietic cells
• Fast, easy-to-use and column-free
• Isolated cells are untouched
- EasySep™ Direct Human CTC Enrichment Kit (Catalog #19657)
- EasySep™ Direct Human CTC Enrichment Cocktail, 2 x 2.5 mL
- EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
Frequently Asked Questions
Can EasySep™ be used for either positive or negative selection?
How does the separation work?
Which columns do I use?
How can I analyze the purity of my enriched sample?
Can EasySep™ separations be automated?
Can EasySep™ be used to isolate rare cells?
Are the EasySep™ magnetic particles FACS-compatible?
Can the EasySep™ magnetic particles be removed after enrichment?
Can I alter the separation time in the magnet?
For positive selection, can I perform more than 3 separations to increase purity?
How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
Data and Publications
Figure 1. Typical EasySep™ Direct Human CTC Enrichment Profile
Starting with human whole blood from healthy donors, spiked with approximately 1% of CAMA cells (epithelial tumor cell line); the typical CTC (EpCAM+) content of non-lysed final enriched fraction is 79 ± 16 % (using the silver “Big Easy” EasySep™ Magnet; gated on DRAQ5™ for nucleated cells). Typically the log depletion of targeted CD45+ cells is 2.8 to 3.2. In the example above, CAMA cells were seeded into whole blood at a starting frequency of 0.98%. The CAMA cell (EpCAM+) content of the enriched fraction is 92.02% with a 3.8 log depletion of CD45+ cells.