EasySep™ Direct Human CTC Enrichment Kit
Immunomagnetic negative selection from whole blood kit

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Overview
Data Figures

Figure 1. Typical EasySep™ Direct Human CTC Enrichment Profile
Starting with human whole blood from healthy donors, spiked with approximately 1% of CAMA cells (epithelial tumor cell line), the typical CTC (epithelial cell+) content of non-lysed final enriched fraction is 79 ± 16 % (using the silver “Big Easy” EasySep™ Magnet; gated on DRAQ5™ for nucleated cells). Typically the log depletion of targeted CD45+ cells is 2.8 to 3.2. In the above example, CAMA cells were seeded into whole blood at a starting frequency of 0.98%. The CAMA cell (epithelial cell+) content of the enriched fraction is 92.02% with a 3.8 log depletion of CD45+ cells.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Resources and Publications
Educational Materials (11)
Frequently Asked Questions
Can EasySep™ be used for either positive or negative selection?
How does the separation work?
Which columns do I use?
How can I analyze the purity of my enriched sample?
Can EasySep™ separations be automated?
Can EasySep™ be used to isolate rare cells?
Are the EasySep™ magnetic particles FACS-compatible?
Can the EasySep™ magnetic particles be removed after enrichment?
Can I alter the separation time in the magnet?
For positive selection, can I perform more than 3 separations to increase purity?
How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
Publications (1)
Abstract
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.