EasySep™ Direct HLA Crossmatch B Cell Isolation Kit

Immunomagnetic negative selection from whole blood, spleen, or lymph nodes kit

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From: 445 USD

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Immunomagnetic negative selection from whole blood, spleen, or lymph nodes kit
From: 445 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Direct HLA Crossmatch B Cell Isolation Kit can be used to isolate highly purified B cells directly from human whole blood, spleen, or lymph nodes by immunomagnetic negative selection. This cell isolation kit targets non-B cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and and are immediately available for downstream applications such as flow cytometry crossmatch.
Advantages:
• > 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis
• Up to 99.8% purity of isolated cells
• Fast, easy-to-use, and column-free
• Isolated cells are untouched
Components:
  • EasySep™ Direct HLA Crossmatch B Cell Isolation Kit (Catalog #19684)
    • EasySep™ Direct HLA Crossmatch B Cell Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
  • EasySep™ Direct HLA Crossmatch B Cell Isolation Kit for RoboSep™ (Catalog #19684RF)
    • EasySep™ Direct HLA Crossmatch B Cell Isolation Cocktail, 2 x 2.5mL
    • EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells
Species:
Human
Sample Source:
Lymph Node; Spleen; Whole Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
HLA; Immunology

Scientific Resources

Product Documentation

Educational Materials

(10)
Brochure
EasySep™ Cell Separation Technology
Brochure
HLA Antibodies for Purity Assessment
Brochure
Cell Separation Products for HLA Analysis
Technical Bulletin
Cell Separation Solutions for the Flow Cytometric Crossmatch Assay
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
1:44
Cell Isolation Directly from Whole Blood without Density Gradient Centrifugation or RBC Lysis: EasySep™ Direct
Video
1:41
RoboSep™-S Introduction
Scientific Poster
Immunomagnetic Cell Isolation of Untouched Lymphocyte Populations Directly from Whole Blood
Scientific Poster
Rapid, Automated Lymphocyte Isolation Directly from Whole Blood with EasySep™ Direct

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
HLA Analysis
Cell Isolation
Characterization

Data and Publications

Data

Starting with human whole blood from normal healthy donors, the B cell (CD19+) content of the non-lysed final isolated fraction typically ranges from 89 - 99.8% (gated on CD45). In the above example, the B cell (CD19+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 12.3% and 98.4% (gated on CD45), respectively.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.