EasySep™ Direct HLA B Cell Isolation Kit (IVD)

Immunomagnetic negative selection from whole blood, buffy coat, spleen, or lymph nodes kit
Catalog #
89684_C
Immunomagnetic negative selection from whole blood, buffy coat, spleen, or lymph nodes kit
Request Pricing
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

The EasySep™ Direct HLA B Cell Isolation Kit (IVD) can be used to isolate highly purified B cells directly from human whole blood, buffy coat, spleen, or lymph nodes by immunomagnetic negative selection.
This kit targets non-B cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and can directly be used for downstream in vitro diagnostic applications.

This product carries the CE mark and is available as a Class I in vitro diagnostic device in the European Union and Australia.

Please Note: In light of the new EU Regulation 2017/746 (IVDR) of the European Parliament and of the Council on in vitro diagnostic medical devices, which is expected to become effective in 2022, STEMCELL has conducted a complete review of our EasySep™ Direct CE-IVD products. We have determined that our current CE-marked IVD EasySep™ Direct products will no longer carry the CE mark as “For In-Vitro Diagnostic Use” and will be labelled “For Research Use Only” labelling. These changes will take effect in December 2021. For more information please contact the Quality Assurance and Regulatory Department at qaschangenote@stemcell.com.
Components
  • EasySep™ Direct HLA B Cell Isolation Kit (Catalog #89684)
    • EasySep™ Direct HLA B Cell Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™ 50302, 4 x 2.5 mL
  • EasySep™ Direct HLA B Cell Isolation Kit for RoboSep™ (Catalog #89684RF)
    • EasySep™ Direct HLA B Cell Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™ 50302, 4 x 2.5 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
    
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #20001)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Lymph Node, Spleen, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
HLA, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Direct HLA B Cell Isolation Kit (IVD)
Catalog #
89684
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Direct HLA B Cell Isolation Kit (IVD)
Catalog #
89684
Lot #
All
Language
Multi
Document Type
Product Information Sheet 1
Product Name
EasySep™ Direct HLA B Cell Isolation Kit for RoboSep™ (IVD)
Catalog #
89684RF
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Direct HLA B Cell Isolation Kit for RoboSep™ (IVD)
Catalog #
89684RF
Lot #
All
Language
Multi
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Direct HLA B Cell Isolation Kit (IVD)
Catalog #
89684
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Direct HLA B Cell Isolation Kit (IVD)
Catalog #
89684
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Direct HLA B Cell Isolation Kit for RoboSep™ (IVD)
Catalog #
89684RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Direct HLA B Cell Isolation Kit for RoboSep™ (IVD)
Catalog #
89684RF
Lot #
All
Language
English

Educational Materials (11)

Brochure
Human B Cell Isolation Product Selection
Brochure
HLA Antibodies for Purity Assessment
Brochure
Cell Separation Products for HLA Analysis
Brochure
EasySep™ Cell Separation Technology
Technical Bulletin
Cell Separation Solutions for the Flow Cytometric Crossmatch Assay
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Video
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
1:44
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
Scientific Poster
Immunomagnetic Cell Isolation of Untouched Lymphocyte Populations Directly from Whole Blood
Scientific Poster
Rapid, Automated Lymphocyte Isolation Directly from Whole Blood with EasySep™ Direct

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Data and Publications

Data

Starting with human whole blood from normal healthy donors, the B cell (CD19+) content of the non-lysed final isolated fraction typically ranges from 89 - 99.8%. In the above example, the B cell (CD19+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 12.3% and 98.4% (gated on CD45), respectively.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.