Dispase, ACF

Animal component-free non-specific protease

Dispase, ACF

Animal component-free non-specific protease

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Animal component-free non-specific protease
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Product Advantages


  • Obtain cleaner cultures with an animal component-free solution

  • Maintain membrane integrity with this mild proteolytic enzyme

  • Use this gentle reagent for isolation and passaging of primary cells


Overview

Use Dispase, Animal Component-Free (ACF) for tissue dissociation and cell isolation procedures. This neutral amino-endoprotease cleaves the N-terminal peptide bond of non-polar amino acid residues, and is obtained from cultures free of animal-derived materials. The mild proteolytic activity of Dispase makes it especially useful for the isolation and passaging of primary cells, and facilitates maintenace of membrane integrity. Dispase is commonly used in combination with other proteases such as collagenase in cell isolation and tissue dissociation for many cell types: neural tissue (Dietrich et al.), kidney (Presnell et al.), epithelial tissue (Smoot et al.), endothelial tissue (Müller et al.), lung (Barkauskas et al.), colon (Roig et al.), and stem cells (Thomson et al.; Salmon et al.).
Subtype
Enzymatic
Alternative Names
Neutral protease; Proteinase
Cell Type
Chondrocytes, Neural Stem and Progenitor Cells, Osteoblasts
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Cell Culture
Area of Interest
Cancer, Endothelial Cell Biology, Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
32.5 kDa

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
Dispase, ACF
Catalog #
100-0396, 07446
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Dispase, ACF
Catalog #
100-0396, 07446
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (1)