Costar® 6.5 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts

Polystyrene plate with lid and polyester membrane inserts

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Costar® 6.5 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts

Polystyrene plate with lid and polyester membrane inserts for cell culture that feed basolaterally

48 Inserts
Catalog #38024
277 USD

Required Products

Overview

Costar® 6.5 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts are recommended for culturing airway epithelial cells at the air-liquid interface in PneumaCult™-ALI Medium (Catalog #05001). These permeable inserts support the growth of both anchorage-dependent and anchorage-independent cells, including those that are fed at the basolateral surface or in co-culture systems.

48 Inserts; 4 x 24-well plates, each with 12 inserts
Application:
Cell Culture

Scientific Resources

Product Documentation

Educational Materials

(3)

Data and Publications

Data

Figure 1. Representative Images of ALI Culture Morphology

A side-by-side comparison was performed between Transwell® Inserts and competitor inserts of the same material and pore size. HBECs were seeded onto the inserts at passage 3 (P3) and differentiated in PneumaCult™-ALI for 21 days. Air-liquid interface (ALI) cultures generated using PneumaCult™ and Transwell® Inserts provided the most optimal morphology.

Figure 2. Differential Epithelial Cell Marker Expression in ALI Cultures Generated with Different Inserts

HBECs were seeded onto the inserts at P3 and differentiated in PneumaCult™-ALI for 21 days. Gene expression of goblet (MUC5B) and ciliated (FOXJ1) cell markers was assessed by qPCR and normalized to beta actin. Comparison was made with competitor inserts of the same material and pore size. Higher expression of goblet and ciliated cell markers was observed when using PneumaCult™ together with Transwell® inserts.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.