Chat with an Expert

ProstaCult™

Defined, Serum-Free Medium for Prostate Epithelial Progenitor Cells

ProstaCult™ is an optimized and standardized medium for the detection, culture and enumeration of mouse prostate epithelial progenitor cells. This defined, serum-free medium is ideal for the culture and evaluation of prostate epithelial progenitor cells in prostate colony-forming unit (Pr-CFU) assays, and enables researchers to detect up to seven times more mouse Pr-CFUs than published formulations. Pr-CFU assays are routinely used to assess prostate epithelial stem cells, which are believed to play a role in prostate cancer. ProstaCult™ can also be used to enzymatically dissociate mouse prostate tissue to a viable single cell suspension.

Mouse prostate epithelial cell colonies cultured in ProstaCult™ Mouse Medium Kit in the presence of an irradiated NIH 3T3 feeder layer can be visualized by Wright-Giemsa staining.

Mouse prostate epithelial cell colonies cultured in ProstaCult™ Mouse Medium Kit in the presence of an irradiated NIH 3T3 feeder layer can be visualized by immunofluorescence staining to detect nuclei (DAPI-blue) and cytokeratin 5 (green).

Why Use ProstaCult™?

  • Detects 7X more prostate colony-forming units than published formulations.
  • Standardized medium and culture conditions increases experimental reproducibility.
  • Minimal lot-to-lot variability reduces experimental variability.
  • Simple two-component system that is combined with cytokines before use.

Prostate Cell Culture Products

ProstaCult™ Mouse Medium Kit

ProstaCult™ Mouse Medium Kit

Recommended for:

Culture and evaluation of mouse prostate epithelial progenitors in the colony-forming unit (CFU) assay

Species:

Mouse
Copyright © 2018 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, AGGREWELL, CELLADHERE, CLONACELL, EASYPLATE, EASYSEP, ENDOCULT, EPICULT, ESCULT, ERYTHROCLEAR, FRESR, MAMMOCULT, MEGACULT, MESENCULT, METHOCULT, MYELOCULT, NEUROCULT, PNEUMACULT, PROSTACULT, RELESR, ROBOSEP, ROSETTESEP, SEPMATE, SMARTDISH, SPINSEP, STEMDIFF, STEMSEP, STEMSPAN, and STEMVISION are trademarks of STEMCELL Technologies Canada Inc. All other trademarks and registered trademarks are the property of their respective holders. While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information.