MesenCult™

MesenCult™

Your High-Performance System for MSC Isolation, Culture & Differentiation

The MesenCult™ product line was developed as a comprehensive and integrated suite of products to help mesenchymal stem cell (MSC) researchers standardize their cell culture system and overcome issues associated with cell culture media variability. Choose from a comprehensive range of specialty products for human and mouse MSCs designed to service scientists along the basic to translational research continuum.

Why Use MesenCult™?

  • Species-optimized media formulations provide superior performance compared to traditional and competitor media.
  • A comprehensive and integrated range of products is available for the enrichment, expansion, quantification (CFU-F Assay) and differentiation of human and mouse mesenchymal stem cells.
  • Standardized media and culture conditions minimize variability and lead to increased reproducibility between experiments.
  • Isolate, culture, cryopreserve and differentiate MSCs with animal component-free, serum-free formulations.

Animal Component-Free Media

Mesenchymal stem cells (MSCs) have potential utility in a range of cellular therapies, with applications relating to tissue engineering and regenerative medicine, and as vehicles for gene therapy. The limited global supply of fetal bovine serum (FBS) and concerns about immune rejection of transplanted cells or disease transmission make animal component-free media an attractive alternative when expanding cells to numbers for therapeutic purposes.

MesenCult™-ACF media together allow for the optimized isolation, clonogenic growth, cryopreservation, long-term expansion and differentiation of human MSCs derived from primary tissues (bone marrow and adipose). To date, this is the only complete and integrated culture system completely free of serum and other materials derived from human or animal sources. With animal component-free media, experimental variability is minimized, and the safety concerns associated with exposure to animal-derived materials in translational research are addressed.

MesenCult™ for Human MSCs

Reduce variation by culturing and differentiating human MSCs in standardized animal component-free, xeno-free or serum-based MesenCult™ media formulations.

MesenCult™-ACF
Culture Kit


MesenCult™-ACF Culture Kit

Product Type:

Defined, serum-free and animal component-free

Applications:

  • MSC isolation and expansion from primary human bone marrow or adipose tissue as well as culture-expanded cells
  • Long-term culture of MSCs (>8 passages)
  • Detection and enumeration of CFU-F from primary human bone marrow or adipose tissue

Features/Advantages:

  • Defined, animal component-free
  • Optimized for isolation of MSC directly from primary human tissue
  • Superior cell expansion compared to serum-containing medium
  • Significant reduction in hematopoietic cell contamination at early passages
  • Supports efficient clonogenic growth and long-term expansion of human MSCs
  • No adaptation required when transitioning MSCs from serum-containing medium

MesenCult™-ACF
Freezing Medium


MesenCult™-ACF Freezing Medium

Product Type:

Defined, serum-free and animal component-free

Applications:

Cryopreservation of human MSCs

Features/Advantages:

  • Defined, serum-free and animal component-free
  • Reproducibly high recovery rates
  • Preserves human MSC multipotency and expansion capacities
  • Convenient, ready-to-use format
  • Optimized for MSCs previously expanded in MesenCult™ (Human), MesenCult™-XF or MesenCult™-ACF

MesenCult™-ACF
Chondrogenic Differentiation Medium


MesenCult™-ACF Chondrogenic Differentiation Medium

Product Type:

Defined, serum-free and animal component-free

Applications:

Animal component-free, in vitro differentiation of MSCs into chondrocytes

Features/Advantages:

  • Defined, animal component-free (ACF) formulation
  • Robust chondrogenic differentiation with as few as 3 x105 MSCs per 3D pellet and as early as day 14
  • Strong expression of chondrogenic transcripts - Sox9, Acan, Col2a and Col10a; low expression of hypertrophic transcript Mmp13
  • Completes optimized ACF workflow for MSC isolation, expansion, cryopreservation and chondrogenic differentiation when used with other MesenCult™-ACF media
  • Compatible with MSCs previously expanded in MesenCult™ (Human), MesenCult™-XF or MesenCult™-ACF
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MesenCult™ for Mouse MSCs

Optimize the culture and differentiation of mouse MSCs with standardized MesenCult™ media and supplements.

MesenCult™
Proliferation Kit with MesenPure™


MesenCult™ Proliferation Kit with MesenPure™

Product Type:

Complete medium for highly enriched mouse mesenchymal stem cell cultures as early as passage 0

Applications:

  • Expansion of mouse MSCs and MEFs
  • Enumeration of mouse MSCs and MEFs using the CFU-F assay

Features/Advantages:

  • Fast enrichment and expansion of mouse MSCs without serial passaging
  • Obtain homogeneous mouse MSC cultures with robust self-regeneration and differentiation potential
  • Optimized for use with bone marrow- and compact bone-derived mouse MSCs
  • Easy to use as MesenPure™ is simply added to complete MesenCult™ medium just prior to use
  • Can also be used for enrichment and expansion of mouse embryonic fibroblasts (MEFs)

MesenCult™
Osteogenic Stimulatory Kit (Mouse)


MesenCult™ Proliferation Kit with MesenPure™

Product Type:

Complete medium for differentiating mouse mesenchymal stem and progenitor cells and embryonic fibroblasts into osteoblasts

Applications:

  • In vitro differentiation of mouse MSCs and mouse embryonic fibroblasts (MEFs) into osteoblasts
  • Bone development studies

Features/Advantages:

  • Achieve unparalleled levels of osteogenic differentiation compared to competitor formulations
  • Superior expression of key osteogenic transcripts involved in bone differentiation and maturation
  • Robust bone matrix deposition (Alizarin Red staining)

MesenCult™
Adipogenic Stimulatory Supplement (Mouse)


MesenCult™ Adipogenic Stimulatory Supplement (Mouse)

Product Type:

Supplement for differentiating mouse mesenchymal stem cells into adipocytes

Applications:

In vitro differentiation of mouse MSCs into adipocytes when used with MesenCult™ MSC Basal Medium (Mouse; Catalog #05501)

Features/Advantages:

  • Compatible with the MesenCult™ Proliferation Kit with MesenPure™ (Mouse; Catalog #05512)
  • Minimal lot-to-lot variability for reproducible results
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Key Applications

Serum-Free Culture of Human Bone-Marrow Derived Mesenchymal Stem Cells

Miwa H et al. (2012) Xeno-free proliferation of human bone marrow mesenchymal stem cells. Cytotechnology 64(3): 301-8

Serum-Free Culture of Human Adipose-Derived Mesenchymal Stem Cells

Other Uses

Serum-Free Culture of Human Placenta-Derived Mesenchymal Stem Cells

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