MesenCult™ Mesenchymal Stromal Cell (MSC) Culture
MesenCult™ Mesenchymal Stromal Cell Culture
Your High-Performance System for MSC Isolation, Culture & Differentiation
The MesenCult™ product line was developed as a comprehensive and integrated suite of products to help mesenchymal stem cell (MSC) researchers standardize their MSC culture system and overcome issues associated with cell culture media variability. Choose from a comprehensive range of specialty products for human and mouse MSCs designed to service scientists along the basic to translational research continuum.
MesenCult™ Workflow for MSC Derivation, Culture & Differentiation
Derive, culture, expand, differentiate, and cryopreserve MSCs using MesenCult™. The MesenCult™ product line offers a range of animal component-free and human platelet lysate- and serum-containing media for MSC research.
Why Use MesenCult™?
- Species-optimized media formulations provide superior performance compared to traditional and competitor media.
- A comprehensive and integrated range of products is available for the enrichment, expansion, quantification (CFU-F Assay) and differentiation of human and mouse mesenchymal stem cells.
- Standardized MSC culture media and conditions minimize variability and lead to increased reproducibility between experiments.
- Isolate, culture, cryopreserve and differentiate human MSCs with animal component-free, serum-free formulations.
MesenCult™ Human MSC Media
Reduce variation by culturing and differentiating human MSCs in standardized serum-free and animal component-free MesenCult™ media formulations.
Animal Component-Free Media
Mesenchymal stem cells (MSCs) have potential utility in a range of cellular therapies, with applications relating to tissue engineering and regenerative medicine, and as vehicles for gene therapy. The limited global supply of fetal bovine serum (FBS) and concerns about immune rejection of transplanted cells or disease transmission make animal component-free media an attractive alternative when expanding cells to numbers for therapeutic purposes.
MesenCult™-ACF media together allow for the optimized isolation, clonogenic growth, cryopreservation, long-term expansion and differentiation of human MSCs derived from primary tissues (bone marrow and adipose). To date, this is the only complete and integrated culture system completely free of serum and other materials derived from human or animal sources. With animal component-free media, experimental variability is minimized, and the safety concerns associated with exposure to animal-derived materials in translational research are addressed.
MesenCult™-ACF Plus Medium
Product Type:
Serum-free and animal component-free
Applications:
- MSC isolation and expansion from primary human bone marrow or adipose tissue as well as culture-expanded cells
- Long-term culture of MSCs (>8 passages)
- Detection and enumeration of CFU-F from primary human bone marrow or adipose tissue
Features/Advantages:
- Animal component-free
- Optimized for isolation of MSC directly from primary human tissue
- Superior cell expansion compared to serum-containing medium
- Significant reduction in hematopoietic cell contamination at early passages
- Supports efficient clonogenic growth and long-term expansion of human MSCs
- No adaptation required when transitioning MSCs from serum-containing medium
MesenCult™-ACF Freezing Medium
Product Type:
Serum-free and animal component-free
Applications:
Cryopreservation of human MSCs
Features/Advantages:
- Serum-free and animal component-free
- Reproducibly high recovery rates
- Preserves human MSC multipotency and expansion capacities
- Convenient, ready-to-use format
- Optimized for MSCs previously expanded in MesenCult™-ACF Plus Medium, MesenCult™-hPL Medium, or MesenCult™ Proliferation Kit (Human)
MesenCult™-ACF Chondrogenic Differentiation Medium
Product Type:
Serum-free and animal component-free
Applications:
Animal component-free, in vitro differentiation of MSCs into chondrocytes
Features/Advantages:
- Animal component-free (ACF) formulation
- Robust chondrogenic differentiation with as few as 3 x105 MSCs per 3D pellet and as early as day 14
- Strong expression of chondrogenic transcripts - Sox9, Acan, Col2a and Col10a; low expression of hypertrophic transcript Mmp13
- Completes optimized ACF workflow for MSC isolation, expansion, cryopreservation and chondrogenic differentiation when used with other MesenCult™-ACF media
- Compatible with MSCs previously expanded in MesenCult™-ACF Plus Medium, MesenCult™-hPL Medium, or MesenCult™ Proliferation Kit (Human)
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Explore More MesenCult™ Products for Human MSCs
Find more products for your human MSC research, including MesenCult™ Osteogenic Differentiation Kit for the differentiation of MSCs into osteoblasts, MesenCult™ Adipogenic Differentiation Kit for the differentiation of MSCs into adipocytes, and MesenCult™ Proliferation Kit for the detection of CFU-F.
MesenCult™ Mouse MSC Media
Optimize the culture and differentiation of mouse MSCs with standardized MesenCult™ media and supplements.
MesenCult™ Expansion Kit (Mouse)
Product Type:
Medium and reagents to expand and enrich for mouse mesenchymal stem and progenitor cultures as early as passage 0
Applications:
- Derivation and expansion of mouse MSCs and MEFs
- Enumeration of mouse MSCs and MEFs using the CFU-F assay
Features/Advantages:
- Fast expansion of mouse MSCs with robust enrichment as early as passage 0
- Optimized for use with mouse bone marrow-, compact bone- and adipose-derived MSCs and MEFs
- Obtain homogeneous mouse MSC cultures while maintaining tri-lineage differentiation potential
- Rigorous raw material screening and quality control minimize lot-to-lot variability
MesenCult™ Osteogenic Stimulatory Kit (Mouse)
Product Type:
Complete medium for differentiating mouse mesenchymal stem and progenitor cells and embryonic fibroblasts into osteoblasts
Applications:
- In vitro differentiation of mouse MSCs and mouse embryonic fibroblasts (MEFs) into osteoblasts
- Bone development studies
Features/Advantages:
- Achieve unparalleled levels of osteogenic differentiation compared to competitor formulations
- Superior expression of key osteogenic transcripts involved in bone differentiation and maturation
- Robust bone matrix deposition (Alizarin Red staining)
MesenCult™ Adipogenic Differentiation Kit (Mouse)
Product Type:
Medium for the differentiation of mouse MSCs, ADSCs, and MEFs into adipocytes
Applications:
- In vitro differentiation of mouse mesenchymal stem and progenitor cells (MSCs), adipose tissue-derived MSCs (ADSCs), and mouse embryonic fibroblasts (MEFs) into cells of the adipogenic lineage
Features/Advantages:
- Compatible with mouse MSCs previously culture-expanded using the MesenCult™ Expansion Kit (Mouse).
- Available in an easy-to-use two-component format.
- Rigorous raw material screening and quality control minimize lot-to-lot variability.
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Scientific Resources
Brochure
Products for Your Mesenchymal Stromal Cell Research
Wallchart
Mesenchymal Stromal Cell-Derived Exosomes: Biogenesis and Cargoes
Wallchart
The Identity and Properties of Mesenchymal Stem Cells
How to Create a Hypoxic Environment for Cell Culture
Learn how to mimic physiological conditions to support MSC growth in vitro with a Hypoxia Incubator Chamber.
MSC Survey Results
Read what 450 researchers from around the world had to say in our survey on the state of the MSC field.
Key Applications
Serum-Free Culture of Human Bone-Marrow Derived Mesenchymal Stem Cells
Miwa H et al. (2012) Xeno-free proliferation of human bone marrow mesenchymal stem cells. Cytotechnology 64(3): 301-8
Serum-Free Culture of Human Adipose-Derived Mesenchymal Stem Cells
García-Contreras M et al. Therapeutic potential of human adipose-derived stem cells (ADSCs) from cancer patients: a pilot study. PLoS One. 9(11):e113288
Other Uses
3D Bioreactor Culture Systems for Mesenchymal Stem Cells
Cunha B et al. (2015) Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells. J Biotechnol doi:10.1016/j.jbiotec.2015.02.023
Tan KY et al. (2015) Serum-free media formulations are cell line-specific and require optimization for microcarrier culture. Cytotherapy 17(8): 1152-65
Hervy M et al. (2014) Long term expansion of bone marrow-derived hMSCs on novel synthetic microcarriers in xeno-free, defined conditions. PLoS One. 9(3):e92120
Tan KY et al. (2015) Serum-free media formulations are cell line-specific and require optimization for microcarrier culture. Cytotherapy 17(8): 1152-65
Hervy M et al. (2014) Long term expansion of bone marrow-derived hMSCs on novel synthetic microcarriers in xeno-free, defined conditions. PLoS One. 9(3):e92120
Serum-Free Culture of Human Umbilical Cord-Derived Mesenchymal Stem Cells
Swamynathan P et al. (2014) Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton's jelly derived mesenchymal stem cells? A comparative study. Stem Cell Res Ther 5(4): 88
Chen G et al. (2014) Human Umbilical Cord-Derived Mesenchymal Stem Cells Do Not Undergo Malignant Transformation during Long-Term Culturing in Serum-Free Medium. PLoS One 9(6):e98565
Chen GC et al. (2014) Monitoring the biology stability of human umbilical cord-derived mesenchymal stem cells during long-term culture in serum-free medium. Cell Tissue Bank 15(4): 513-21
Zhou P et al. (2013) Serum-free culture of umbilical cord-derived mesenchymal stem cells. Zhongquo Shi Yan Xue Ye Xue Za Zhi 21(5): 1256-60
Chen G et al. (2014) Human Umbilical Cord-Derived Mesenchymal Stem Cells Do Not Undergo Malignant Transformation during Long-Term Culturing in Serum-Free Medium. PLoS One 9(6):e98565
Chen GC et al. (2014) Monitoring the biology stability of human umbilical cord-derived mesenchymal stem cells during long-term culture in serum-free medium. Cell Tissue Bank 15(4): 513-21
Zhou P et al. (2013) Serum-free culture of umbilical cord-derived mesenchymal stem cells. Zhongquo Shi Yan Xue Ye Xue Za Zhi 21(5): 1256-60
Serum-Free Culture of Human Placenta-Derived Mesenchymal Stem Cells
Zhu Y et al. (2015) Alteration of Histone Acetylation Pattern during Long-Term Serum-Free Culture Conditions of Human Fetal Placental Mesenchymal Stem Cells PLoS One 10(2): e0117068
