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HepatiCult™

Complete, Defined Medium for Hepatic Organoid Culture

HepatiCult™ Organoid Growth Medium (Mouse) is a serum-free, defined cell culture medium for establishing and maintaining mouse hepatic progenitor organoids. These organoids, or "mini-livers", provide an in vitro culture system for studying hepatic stem and progenitor cells. Hepatic organoids can be maintained through passaging every 4 - 7 days or cryopreserved, and are primed for downstream differentiation. HepatiCult™ supports mouse hepatic organoid culture either embedded in Corning® Matrigel® domes or in a dilute Matrigel® suspension.

Using Organoids as Model Systems

Growing hepatic progenitors as organoid cultures enables convenient in vitro characterization of the hepatic epithelium in a physiologically relevant system. In this webinar, Dr. Meritxell Huch discusses organoid culture and their applications in modeling the GI tract.

Why Use HepatiCult™?

  • In vitro system for generating organoids within a week.
  • No injury models, hand-picking of ducts or cell sorting required.
  • Serum-free and defined medium formulation.
  • Organoids can be grown from duct fragments or single cells and be cultured in matrix domes or suspension.

Products for Hepatic Organoid Culture

HepatiCult™ Organoid Growth Medium

Recommended for:

Robust establishment and maintenance of hepatic stem cells in Matrigel® domes or in a dilute Matrigel® suspension

Species:

Mouse

Components:

  • HepatiCult™ OGM Mouse Basal Medium, 95 mL
  • HepatiCult™ OGM Mouse Supplement, 5 mL

Resources:

Mouse Hepatic Organoids

Recommended for:

Convenient establishment and standardization of hepatic organoid cultures in the laboratory

Species:

Mouse

Contents:

Frozen mouse hepatic progenitor organoid segments

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Brand History

Despite the remarkable regenerative capacity of the liver in vivo, sustained in vitro culture of liver tissues has remained difficult until recently. In 2013, Dr. Meritxell Huch et al. described a robust culture system for the maintenance of hepatic tissues as organoids. In 2014, Dr. Hans Clevers and the Foundation Hubrecht Organoid Technology signed an agreement with STEMCELL Technologies to manufacture and distribute cell culture media for organoids. HepatiCult™ Organoid Growth Medium (Mouse) was released in early 2018, providing researchers with a convenient method for establishing and maintaining hepatic progenitor organoids.


Key Applications of Hepatic Organoids

Disease Modeling and Cancer Research

Hepatic Cell Biology

Dorrell C, et al. (2014) The organoid-initiating cells in mouse pancreas and liver are phenotypically and functionally similar. Stem Cell Res. 13(2): 275-83.

Tarlow BD, et al. (2014) Clonal tracing of Sox9+ liver progenitors in mouse oval cell injury. Hepatology. 60(1): 278-89.

Yimlamai D, et al. (2014) Hippo pathway activity influences liver cell fate. Cell. 157(6): 1324-38.

Kruitwagen HS, et al. (2018) DYRK1A Is a Regulator of S-Phase Entry in Hepatic Progenitor Cells. Stem Cells Dev.

Organoids in Multi-Lineage Model Systems

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