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Applications of the Hematopoietic Progenitor Assay Training Course

Three-day hands-on training on the CFU Assay for human, mouse, and rat samples

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1,750 USD

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Three-day hands-on training on the CFU Assay for human, mouse, and rat samples
1,750 USD

Course Overview

This course focuses on hematopoietic progenitor assays for human, mouse and rat samples with an emphasis on applying the colony-forming unit (CFU) assay to your research. Participants will gain confidence in performing the CFU assay, identifying and enumerating hematopoietic progenitor cells. The use of the CFU assay in drug development and predictive toxicity testing is also discussed. Participants will learn how to effectively design in vitro and in vivo assays to understand the effects of biological pathways on hematopoietic homeostasis and also how to evaluate the effect of compounds on blood and bone marrow using various mouse models.


Participants Will Receive
  • Course booklet and electronic versions of instructional material
  • Tote bag with sample-size products (where applicable)
  • Certificate following successful completion of the course
  • Lunch and refreshments on each day and dinner hosted on one of the evenings
Training and Education
Training Programs
Stem Cell Biology; Drug Discovery and Toxicity Testing; Hematologic Malignancies
Hematopoietic Stem and Progenitor Cells
Human, Mouse, and Rat
Canada:
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6
United Kingdom:
STEMCELL Technologies UK Ltd.
Building 7100, Cambridge Research Park,
Beach Drive, Waterbeach
Cambridge, UK, CB25 9TL

Speakers

Jackie Damen
Dr. Jackie Damen
Scientific Director of the Contract Assay Services
STEMCELL Technologies

Dr. Jackie Damen is the Scientific Director of the Contract Assay Services department. In a career spanning over 20 years, Dr. Damen has acquired extensive experience in the field of hematopoiesis, where her key strengths lie in stem cell biology, protein biochemistry and signalling, especially in the area of erythropoietin. She manages all aspects of the Contract Assay Services department which includes client consultation and customized experimental design, execution and analysis. Dr. Damen actively participates in several educational initiatives, regularly providing online talks and presentations of topical interest at high profile conferences such as the Society of Toxicology Annual Meeting. At STEMCELL Technologies, Dr. Damen has been involved in customer training of hematopoietic stem cell biology and colony identification since 2002.

Raymond Lam
Raymond Lam
Manager, Scientific Knowledge, Education & Product and Scientific Support
STEMCELL Technologies

Raymond Lam is the Manager of Scientific Knowledge, Europe. Raymond joined STEMCELL in 2004 as a Quality Control Analyst, where he was responsible for the testing of all finished products. He then transferred to the Product and Scientific Support department to directly respond to customer inquiries on protocol and products. In 2008, Raymond became a Field Applications Specialist, supporting customers in the field as well as conducting training and seminars at academic institutions. In his current role, Raymond oversees the expansion and standardization of educational training programs globally for staff, customers, and distributors on STEMCELL Technologies’ products and workflows.

Course Agenda (Tentative)

Day One

  • Welcome Presentation and Introductions
  • Lecture: Introduction to Hematopoiesis and the CFU Assay
  • Lecture 2: CFU Assay Set Up and Colony Identification
  • Practical Session 1 : CFU Assay Set Up Procedure
    • Incubator Maintenance
    • Sample Processing with ErythroClear™ Red Blood Cell Depletion Kit
    • CFU Assay Setup
    • Demo Sample Thawing and Cell Counting
  • Practical Session 2: Manual Colony Identification for Human Samples
  • Practical Session 3: Comparative Colony Counting for Human Samples
  • Wrap Up and Q&A

Day Two

  • Lecture: Introduction to Hematopoiesis and Standardization of the CFU Assay Set Up for Mouse and Other Species
  • Lecture: CFU Assay for Megakaryocytic Progenitors
  • Practical Session: Review of Manual Colony Identification Criteria and Comparative Counting for Human Samples
  • Practical Session: Sample Processing and Colony Identification for Mouse Samples
  • Practical Session: MegaCult™ CFU-Mk Assay
    • Assay Set Up
    • Colony Identification and Counting
  • Practical Session: Colony Identification for Mouse and Rat Samples
  • Wrap Up and Q&A

Day Three

  • Lecture: Applications of Hematopoietic CFU Assay for Mouse and Other Species
  • Lecture: Serum-Free Hematopoietic Cell Expansion Supplements and HemaTox™ Kits
  • Practical Session: Review of Manual Colony Identification Criteria and Comparative Counting for Mouse Samples
  • Practical Session:
    • Review of Manual Colony Identification Criteria & Individual Comparative Counting for Other Species
    • Set Up Toxicity Curve and Observe Cultures
  • Final Wrap Up:
    • Q&A
    • Quiz
    • Course Evaluation
    • Certificate Presentation

Accommodations

Vancouver, Canada

Pan Pacific Vancouver
999 Canada Pl #300, Vancouver, BC, V6C 3B5
Tel 1-800-663-1515 or 1-604-662-3223
Sheraton Wall Centre
1088 Burrard St. Vancouver, BC V6Z2R9
Tel: 1-604-331-1000
Holiday Inn Vancouver-Centre
711 West Broadway, Vancouver, BC V5Z 3Y2
Tel: 1-604-879-0511
Executive Hotel LeSoleil
567 Hornby St, Vancouver, BC V6C 2E8
Tel: 1-604-632-3000

Cambridge, UK

Holiday Inn Cambridge
Lakeview, Bridge Rd, Impington, Cambridge CB24 9PH, UK
Five minute taxi ride to course location.

Contact education@stemcell.com for information on special rates (when applicable).

Policy

Travel Visas

Citizens may require a visa in order to enter the country as visitors. Please contact us at education@stemcell.com if you require a letter of invitation for your application. Note that travel visas may take up to 30 days to process.

Travel Visa Requirements

Canada
United Kingdom

Cancellation

A 90% refund will be issued if notification is received in writing more than 30 days in advance of the course start date. No refund will be given with less than 30 days’ notice unless we are able to find another participant to fill the spot.

STEMCELL Technologies reserves the right to cancel the course if we do not receive a minimum number of registrations 30 days in advance of the start date. Should the course be cancelled, your payment will be refunded in full. We recommend that you purchase travel insurance with your flights to cover the possibility of the course being cancelled.

Scientific Resources

Educational Materials

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