This course focuses on hematopoietic progenitor assays for human, mouse and rat samples with an emphasis on applying the colony-forming unit (CFU) assay to your research. Participants will gain confidence in performing the CFU assay, identifying and enumerating hematopoietic progenitor cells. The use of the CFU assay in drug development and predictive toxicity testing is also discussed. Participants will learn how to effectively design in vitro and in vivo assays to understand the effects of biological pathways on hematopoietic homeostasis and also how to evaluate the effect of compounds on blood and bone marrow using various mouse models.
Participants Will Receive
- Course booklet and electronic versions of instructional material
- Tote bag with sample-size products (where applicable)
- Certificate following successful completion of the course
- Lunch and refreshments on each day and dinner hosted on one of the evenings
1618 Station Street
Vancouver, CA V6A 1B6
STEMCELL Technologies UK Ltd.
Building 7100, Cambridge Research Park,
Beach Drive, Waterbeach
Cambridge, UK, CB25 9TL
Dr. Jackie Damen is the Scientific Director of the Contract Assay Services department. In a career spanning over 20 years, Dr. Damen has acquired extensive experience in the field of hematopoiesis, where her key strengths lie in stem cell biology, protein biochemistry and signalling, especially in the area of erythropoietin. She manages all aspects of the Contract Assay Services department which includes client consultation and customized experimental design, execution and analysis. Dr. Damen actively participates in several educational initiatives, regularly providing online talks and presentations of topical interest at high profile conferences such as the Society of Toxicology Annual Meeting. At STEMCELL Technologies, Dr. Damen has been involved in customer training of hematopoietic stem cell biology and colony identification since 2002.
Raymond Lam is the Manager of Scientific Knowledge, Europe. Raymond joined STEMCELL in 2004 as a Quality Control Analyst, where he was responsible for the testing of all finished products. He then transferred to the Product and Scientific Support department to directly respond to customer inquiries on protocol and products. In 2008, Raymond became a Field Applications Specialist, supporting customers in the field as well as conducting training and seminars at academic institutions. In his current role, Raymond oversees the expansion and standardization of educational training programs globally for staff, customers, and distributors on STEMCELL Technologies’ products and workflows.
Course Agenda (Tentative)
Welcome Presentation and Introductions
Lecture: Introduction to Hematopoiesis and the CFU Assay
Lecture 2: CFU Assay Set Up and Colony Identification
Practical Session 1 : CFU Assay Set Up Procedure
- Incubator Maintenance
- Sample Processing with ErythroClear™ Red Blood Cell Depletion Kit
- CFU Assay Setup
- Demo Sample Thawing and Cell Counting
Practical Session 2: Manual Colony Identification for Human Samples
Practical Session 3: Comparative Colony Counting for Human Samples
Wrap Up and Q&A
Lecture: Introduction to Hematopoiesis and Standardization of the CFU Assay Set Up for Mouse and Other Species
Lecture: CFU Assay for Megakaryocytic Progenitors
Practical Session: Review of Manual Colony Identification Criteria and Comparative Counting for Human Samples
Practical Session: Sample Processing and Colony Identification for Mouse Samples
Practical Session: MegaCult™ CFU-Mk Assay
- Assay Set Up
- Colony Identification and Counting
Practical Session: Colony Identification for Mouse and Rat Samples
Wrap Up and Q&A
Lecture: Applications of Hematopoietic CFU Assay for Mouse and Other Species
Lecture: Serum-Free Hematopoietic Cell Expansion Supplements and HemaTox™ Kits
Practical Session: Review of Manual Colony Identification Criteria and Comparative Counting for Mouse Samples
- Review of Manual Colony Identification Criteria & Individual Comparative Counting for Other Species
- Set Up Toxicity Curve and Observe Cultures
Final Wrap Up:
- Course Evaluation
- Certificate Presentation
Tel 1-800-663-1515 or 1-604-662-3223
Five minute taxi ride to course location.
Contact firstname.lastname@example.org for information on special rates (when applicable).