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Anti-Mouse CD25 Antibody, Clone PC61.5

Rat monoclonal IgG1 antibody against mouse CD25

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Anti-Mouse CD25 Antibody, Clone PC61.5

Rat monoclonal IgG1 antibody against mouse CD25

From: 60 USD
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Rat monoclonal IgG1 antibody against mouse CD25
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Overview

The PC61.5 antibody reacts with mouse CD25 (low-affinity interleukin‐2 receptor α chain or IL-2Rα), which is an ~55 kDa type 1 transmembrane glycoprotein expressed on T and B cell progenitors, activated (but not resting) T and B cells, regulatory T (Treg) cells, dendritic cells, and activated monocytes and macrophages. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. CD25 per se has low affinity for its IL-2 ligand but associates with CD122 (IL-2Rβ) and CD132 (IL-2Rγ) to form the high-affinity IL-2R receptor. CD25 is necessary for receptor clustering and induction of signaling pathways involved in the activation and proliferation of lymphocytes. Binding of the PC61.5 antibody inhibits binding of IL-2 to the low- and high-affinity receptors, which may result in inhibition of IL-2-dependent proliferation. The epitope recognized by PC61.5 is distinct from the IL-2 binding site and from the epitopes recognized by antibodies 7D4 and 3C7.
Subtype
Primary Antibodies
Target Antigen
CD25
Alternative Names
IL-2 receptor subunit alpha, IL-2Ra, IL-2Ralpha, IL-2-RA, Interleukin-2 receptor subunit alpha, Ly-43, p55, Tac
Reactive Species
Mouse
Conjugation
APC, Biotin, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
T Cells, T Cells, Regulatory
Species
Mouse
Application
Depletion, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Neutralization and Blocking
Area of Interest
Immunology
Clone
PC61.5
Gene ID
16184
Isotype
IgG1, lambda

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071 (Catalog #60075), followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled withRat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC (Catalog #60075AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PE and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE (Catalog #60075PE) and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Figure 4. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with a rat IgG1 isotype control antibody, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a biotinylated rat IgG1 isotype control antibody followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5
Catalog #
60009
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin
Catalog #
60009BT.1, 60009BT.2, 60009BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, APC
Catalog #
60009AZ.1, 60009AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5
Catalog #
60009PS.1, 60009PS
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PE
Catalog #
60009PE.1, 60009PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5
Catalog #
60009
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin
Catalog #
60009BT.1, 60009BT.2, 60009BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, APC
Catalog #
60009AZ.1, 60009AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5
Catalog #
60009PS.1, 60009PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PE
Catalog #
60009PE.1, 60009PE
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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