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Anti-Mouse CD24 Antibody, Clone M1/69

Rat monoclonal IgG2b antibody against mouse CD24

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Anti-Mouse CD24 Antibody, Clone M1/69

Rat monoclonal IgG2b antibody against mouse CD24

From: 83 USD
Catalog #
60099_C
Rat monoclonal IgG2b antibody against mouse CD24
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Overview

The M1/69 antibody reacts with murine CD24, an ~35 - 45 kDa glycoprotein expressed on lymphocytes, granulocytes, thymocytes, erythrocytes, epithelial cells, neurons, and dendritic cells. CD24 is differentially expressed during T and B cell development, and is not found on the majority of mature peripheral T cells or plasma cells. It is expressed on pro-B, pre-B and mature B cells, but expression is strongly down-regulated after B cell activation. CD24 is involved in regulating B cell apoptosis and in preventing terminal differentiation of B cells into antibody-secreting plasmablasts and plasma cells. It acts as an adhesion or co-stimulatory molecule and is involved in lymphocyte activation, proliferation and differentiation through homophilic interactions or by binding to ligands such as P-selectin (CD62P).
Subtype
Primary Antibodies
Target Antigen
CD24
Alternative Names
CD24a, Heat stable antigen, HSA, Ly-52, Nectadrin, Small cell lung carcinoma cluster 4 antigen
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
B Cells, Mammary Cells
Species
Mouse
Application
CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blotting
Area of Interest
Epithelial Cell Biology, Immunology
Clone
M1/69
Gene ID
12484
Isotype
IgG2b, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, Alexa Fluor® 488 (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (Catalog #60077AD) (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC (solid line histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, PE (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (Catalog #60077PE) (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Data for APC-Conjugated

Figure 6. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Data for FITC-Conjugated

Figure 7. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, PE
Catalog #
60099PE.1, 60099PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, PerCP-Cy5.5
Catalog #
60099PS.1, 60099PS
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, FITC
Catalog #
60099FI, 60099FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, APC
Catalog #
60099AZ, 60099AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, Alexa Fluor® 488
Catalog #
60099AD.1, 60099AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, Biotin
Catalog #
60099BT, 60099BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69
Catalog #
60099.1, 60099
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, PE
Catalog #
60099PE.1, 60099PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, PerCP-Cy5.5
Catalog #
60099PS.1, 60099PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, FITC
Catalog #
60099FI, 60099FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, APC
Catalog #
60099AZ, 60099AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, Alexa Fluor® 488
Catalog #
60099AD.1, 60099AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, Biotin
Catalog #
60099BT, 60099BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69
Catalog #
60099.1, 60099
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

Publications (1)

Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function Pond AC et al. Stem cells (Dayton, Ohio) 2013

Abstract

Fibroblast growth factor (FGF) signaling plays an important role in embryonic stem cells and adult tissue homeostasis, but the function of FGFs in mammary gland stem cells is less well defined. Both FGFR1 and FGFR2 are expressed in basal and luminal mammary epithelial cells (MECs), suggesting that together they might play a role in mammary gland development and stem cell dynamics. Previous studies have demonstrated that the deletion of FGFR2 resulted only in transient developmental defects in branching morphogenesis. Using a conditional deletion strategy, we investigated the consequences of FGFR1 deletion alone and then the simultaneous deletion of both FGFR1 and FGFR2 in the mammary epithelium. FGFR1 deletion using a keratin 14 promoter-driven Cre-recombinase resulted in an early, yet transient delay in development. However, no reduction in functional outgrowth potential was observed following limiting dilution transplantation analysis. In contrast, a significant reduction in outgrowth potential was observed upon the deletion of both FGFR1 and FGFR2 in MECs using adenovirus-Cre. Additionally, using a fluorescent reporter mouse model to monitor Cre-mediated recombination, we observed a competitive disadvantage following transplantation of both FGFR1/R2-null MECs, most prominently in the basal epithelial cells. This correlated with the complete loss of the mammary stem cell repopulating population in the FGFR1/R2-attenuated epithelium. FGFR1/R2-null MECs were partially rescued in chimeric outgrowths containing wild-type MECs, suggesting the potential importance of paracrine mechanisms involved in the maintenance of the basal epithelial stem cells. These studies document the requirement for functional FGFR signaling in mammary stem cells during development.
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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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