Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J

Mouse monoclonal IgG1 antibody against human, chimpanzee, gibbon TRA-2-54 (tissue non-specific alkaline phosphatase)

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Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J

Mouse monoclonal IgG1 antibody against human, chimpanzee, gibbon TRA-2-54 (tissue non-specific alkaline phosphatase), unconjugated

100 µg
Catalog #60067
398 USD

Overview

The TRA-2-54/2J antibody reacts with the tissue non-specific isoform of alkaline phosphatase (AP), which is expressed at high levels in undifferentiated pluripotent stem cells such as human and mouse embryonic stem (ES) cells, as well as induced pluripotent stem (iPS) and embryonal carcinoma (EC) cells. This isozyme of AP is the same as that expressed by liver, bone and kidney cells. Levels of AP decrease upon differentiation and, thus, the TRA-2-54/2J antibody can be used to monitor the differentiation status of pluripotent stem cells. The TRA-2-54/2J antibody does not cross-react with other isozymes of AP, which differ through post-translational modification, and reportedly does not recognize AP from cow, dog, goat, Guinea pig, hamster, mouse, rat or sheep, or Old World Monkeys such as the rhesus monkey, baboon and African green monkey.
Subtype:
Primary Antibodies
Target Antigen:
TRA-2-54
Alternative Names:
Liver/bone/kidney alkaline phosphatase, tissue non-specific alkaline phosphatase
Reactive Species:
Cat; Chimpanzee; Human; Pig; Rabbit; Non-Human Primate
Conjugation:
Unconjugated
Host Species:
Mouse
Cell Type:
Pluripotent Stem Cells
Application:
Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Western Blotting
Area of Interest:
Stem Cell Biology
Clone:
TRA-2-54/2J
Gene ID:
249
Isotype:
IgG1, kappa

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) labeled with Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI). Labeling of human ES cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by Goat Anti-Mouse IgG (H+L), Polyclonal, FITC is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody, followed by goat anti-mouse IgG, FITC. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram). (D) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (negative control, lane 2) with Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.